Home
NCK1
Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6)

Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6) (CAT#: VS3-FY1008)

Name: Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6)
SKU: VS3-FY1008
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes NCK1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R09-7F6 is specific for human, mouse, rat NCK1. The antigen is human nck synthetic peptide.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1. Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6) in ICC.

Figure 1. Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6) in ICC.

Immunocytochemical analysis of NCK1 (green) in HeLa using NCK1 antibody and DAPI (blue).

Figure 2. Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6) in WB.

Figure 2. Recombinant Rabbit Anti-NCK1 Antibody (clone R09-7F6) in WB.

Western Blot analysis of Nck in K562, rat brain, C6, 3T3, Hela lysates using Nck antibody.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30766/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_B_8_5.jpg

Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_A_8_3.jpg

Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_A_7_4.jpg

Figure 5 Kidney
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_A_7_5.jpg

Figure 6 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_A_6_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5063/17857_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000158092-NCK1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human Nck Synthetic Peptide.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat NCK1

Species Reactivity
Human, Mouse, Rat

Clone
R09-7F6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 43 kDa; Observed MW: 43 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
NCK Adaptor Protein 1; SH2/SH3 Adaptor Protein NCK-Alpha; Nck-1; NCK; Non-Catalytic Region Of Tyrosine Kinase; Cytoplasmic Protein NCK1; Melanoma NCK Protein; NCK Tyrosine Kinase; NCKalpha

Gene ID
4690

UniProt ID
P16333

Secondary Antibody

Isotype Control

Related Resources

Related Diseases
Related Signaling Pathways

Bladder Cancer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "NCK1"

Select a product category from the dropdown menu below to view related products.

Please select product type

IgG Antibody Products Rabbit Monoclonal Antibody Products Non-Human Primate (NHP) Antibody Products

CAT	Product Name	Application	Type
MOB-2306z	Mouse Anti-NCK1 Recombinant Antibody (clone 26G5)	WB, ELISA, FC, IHC	Mouse IgG1
MOB-0024CT	Recombinant Mouse anti-Human NCK1 Monoclonal antibody (EML555)	WB
MRO-1065-CN	Recombinant Rabbit Anti-NCK1 Monoclonal Antibody (JU41-80)	WB, IF, IHC, IP, FC	Rabbit IgG
VS3-XY1143	Mouse Anti-NCK1 Recombinant Antibody (clone 5B7)	ELISA, WB, IHC, FC	Mouse IgG1

Customer Reviews and Q&As

There are currently no Customer reviews or questions for VS3-FY1008. Click the button above to contact us or submit your feedback about this product.

Popular products with customers

Figure 1 Anti-ErbB2 Antibody (TAB-005) in WB

Anti-Human ErbB2 Recombinant Antibody (TAB-005) (CAT# TAB-005)
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC

Figure 1 Anti-Human IL12+IL23 Recombinant Antibody (TAB-063) in SDS-PAGE

Anti-Human IL12+IL23 Recombinant Antibody (Ustekinumab) (CAT# TAB-063)
Application: WB, FuncS, IF, Neut, ELISA, FC, IP

Figure 1 Anti-Human NGF Recombinant Antibody (TAB-111) in DB

Anti-Human NGF Recombinant Antibody (TAB-111) (CAT# TAB-111)
Application: ELISA, FuncS, Inhib

Figure 1 Anti-Human VEGF Recombinant Antibody (TAB-011) in WB

Anti-Human VEGF Recombinant Antibody (Bevacizumab) (CAT# TAB-011)
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC

Figure 1 Anti-Human EPCAM Antibody (TAB-235CL) in ELISA

Human Anti-Human EPCAM Recombinant Antibody (3622W94) (CAT# TAB-235CL)
Application: Cyt, FC

Figure 1 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in SDS-PAGE

Human Anti-HIV-1 Env Recombinant Antibody (clone PGDM1400) (CAT# PABL-151)
Application: Neut, FuncS

Figure 1 Human Anti-HIV-1 gp41 Recombinant Antibody in SDS-PAGE analysis.

Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5) (CAT# PABL-186)
Application: WB, Neut, FuncS

Figure 1 Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) in SDS-PAGE

Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) (CAT# PABL-202)
Application: WB, ELISA, FuncS

Figure 1 Mouse Anti-His Tag Recombinant Antibody Fab Fragment (PFBL-134) in SDS-PAGE

Mouse Anti-His Tag Recombinant Antibody (clone YC103); Fab Fragment (CAT# PFBL-134)
Application: WB, ELISA, FuncS

Figure 1 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in SDS-PAGE

Mouse Anti-GM3 Recombinant Antibody (clone 14F7) (CAT# PABC-051)
Application: ELISA

Figure 1 Anti-CD3 Antibody (TAB-0416CL) Induced PBMCs Proliferation

Mouse Anti-CD3 Recombinant Antibody (TAB-0416CL) (CAT# TAB-0416CL)
Application: FC, IB, ELISA

Figure 1 Human Anti-CD3 Recombinant Antibody (TAB-0416CL-S(P)) in SDS-PAGE

Mouse Anti-CD3 Recombinant Antibody scFv Fragment (TAB-0416CL-S(P)) (CAT# TAB-0416CL-S(P))
Application: IB, ELISA, FC, FuncS

Figure 1 Anti-Human TfR Recombinant Antibody (TAB-628CT) in SDS-PAGE

Anti-Human TfR Recombinant Antibody (3GH7 ) (CAT# TAB-628CT)
Application: WB

Figure 1 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in SDS-PAGE

Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) (CAT# TAB-664CT)
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF

Figure 1 Anti-Human ERBB2 Recombinant Antibody (TAB-558MZ) in SDS-PAGE

Human Anti-NOTCH3 Recombinant Antibody (TAB-558MZ) (CAT# TAB-558MZ)
Application: Inhib, Neut

Figure 1 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in ELISA.

Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (CAT# MRO-961CQ)
Application: ELISA

Figure 1 Anti-Human F8 Antibody in SDS-PAGE

Recombinant Human Anti-F8 Antibody (BO2C11) (CAT# PABX-058)
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR

Figure 1 Anti-Human SELP Protein Antibody in ELISA

Anti-Human SELP Recombinant Antibody (TAB-015ML) (CAT# TAB-015ML)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 1 Mouse Anti-CD47 Recombinant Antibody (clone MABL-1) (HPAB-1278WJ) in SDS-PAGE

Mouse Anti-CD47 Recombinant Antibody (clone MABL-1) (CAT# HPAB-1278WJ)
Application: ELISA, Cyt, PP, Inhib

Figure 1 Human Anti-IAV HA Recombinant Antibody (PABJ-0204) in SDS-PAGE

Human Anti-IAV HA Recombinant Antibody (PABJ-0204) (CAT# PABJ-0204)
Application: ELISA, Inhib, FuncS

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry