Recombinant Rabbit Anti-Nefl Antibody (clone R04-7H3) (CAT#: VS3-FY1025)
This product is a recombinant rabbit antibody that recognizes Nefl. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R04-7H3 is specific for mouse Nefl. The antigen is mouse 68kda neurofilament recombinant protein.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
Figure 2 Low expression in tonsil
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
(Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
Figure 4 RNA cell line category: Group enriched (HEK93, PC-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
Specifications
- Immunogen
- mouse 68kDa neurofilament recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse Nefl
- Species Reactivity
- Mouse
- Clone
- R04-7H3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 62 kDa; Observed MW: 68 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Neurofilament Light; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament, Light Polypeptide 68kDa; Neurofilament Triplet L Protein; NF-L; NF68; NFL; Light Molecular Weight Neurofilament Protein; Neurofilament Protein, Light Chain
- Gene ID
- 4747
- UniProt ID
- P07196
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament
- Post Translation Modifications
- O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.
- Protein Refseq
- NP_006149.2
- Function
- Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NEFL"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2006z | Mouse Anti-NEFL Recombinant Antibody (clone 32C12) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1158CT | Recombinant Mouse anti-Rat NEFL Monoclonal antibody (2E3) | ICC/IF, IHC-Fr, IHC-P, WB | |
| ZG-0563F | Mouse Anti-NEFL Recombinant Antibody (clone 2F11) | IHC-Fr, IHC-P, IB | Mouse IgG1 |
| VS3-QX806 | Mouse Anti-NEFL Recombinant Antibody (clone 1H3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1026 | Recombinant Mouse Anti-NEFL Antibody (clone 2D7-6C10-10B2) | IHC-P | Mouse IgG2b |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0822MZ | Chicken Anti-NEFL Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1153MZ | Chicken Anti-Neurofilament Light Polyclonal IgY | WB, ICC | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2411 | Hi-Affi™ Recombinant Rabbit Anti-NEFL Monoclonal Antibody (DS2411AB) | WB, IP, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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