Recombinant Rabbit Anti-Nr3C1 Antibody (clone R04-8C8)
CAT#: VS3-FY1067
This product is a recombinant rabbit antibody that recognizes Nr3C1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R04-8C8 is specific for human, mouse, rat Nr3C1. The antigen is mouse glucocorticoid receptor recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4248/78_E8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/if_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_7_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_8_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10435/26792_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113580-NR3C1
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WB
Figure 1. Recombinant Rabbit Anti-Nr3C1 Antibody (clone R04-8C8) in WB.
Western Blot analysis of Glucocorticoid Receptor in K562, 3T3, Hela lysates using Glucocorticoid Receptor Antibody.
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Specifications
- Immunogen
- mouse glucocorticoid receptor recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat Nr3C1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-8C8
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 87 kDa; Observed MW: 94,91 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NR3C1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1761z | Mouse Anti-NR3C1 Recombinant Antibody (clone 20F1) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-0664-CN | Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (JF0952) | WB, FC | Rabbit IgG |
| VS3-XY1182 | Mouse Anti-NR3C1 Recombinant Antibody (clone 6E6) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-FY1960 | Rabbit Anti-NR3C1 Recombinant Antibody (clone R02-5A3) | WB | Rabbit IgG |
| VS3-WK2028 | Rabbit Anti-NR3C1 (pS226) Recombinant Antibody (clone R02-1H0) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1788CQ | Recombinant Mouse Anti-NR3C1 Antibody (BuGR2) | WB, Neut, ChIP, FC, GS, ICC, IF, IHC, IHC-P, IP | IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2493 | Hi-Affi™ Recombinant Rabbit Anti-NR3C1 Monoclonal Antibody (DS2493AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC345 | AbPlus™ Anti-NR3C1 Magnetic Beads (VS-0724-YC345) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC840 | CytoStream™ Mouse Anti-NR3C1 Recombinant Antibody (VS13-YC840) | WB, IHC-P, FC, ICC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1514 | Anti-NR3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4960 | Anti-Mouse NR3C1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4959 | Anti-Human NR3C1 Immunohistochemistry Kit | IHC |
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