Recombinant Rabbit Anti-PAH Antibody (clone 17D10) (CAT#: VS4-CJ66)
This rabbit anti-PAH antibody (clone 17D10) is a product that recognizes human PAH. The immunogen of this antibody is a synthesized peptide derived from human PAH. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28407/1399_C5_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28407/1399_C5_1_selected.jpg
Figure 2 High expression in liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_9_4.jpg
Figure 3 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_5_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_5_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171759-PAH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171759-PAH
Specifications
- Immunogen
- A synthesized peptide derived from human PAH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PAH
- Species Reactivity
- Human
- Clone
- 17D10
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- PAH; PH; PKU; PKU1
- Gene ID
- 5053
- UniProt ID
- P00439
- Long Name
- Phenylalanine hydroxylase
- Sequence Similarities
- Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.
- Protein Refseq
- NP_000268.1
- Function
- Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1959z | Mouse Anti-PAH Recombinant Antibody (clone 36C11) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| VS3-QX848 | Mouse Anti-PAH Recombinant Antibody (clone 717CT21.1.1) | WB, IHC | Mouse IgG1, κ |
| VS3-CJ204 | Mouse Anti-PAH Recombinant Antibody (VS3-CJ204) | WB, IHC-P | Mouse IgG1, κ |
| VS3-FY1613 | Rabbit Anti-PAH Recombinant Antibody (clone R06-3H4) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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