Recombinant Rabbit Anti-PAH Antibody (clone 17D10)
CAT#: VS4-CJ66
This rabbit anti-PAH antibody (clone 17D10) is a product that recognizes human PAH. The immunogen of this antibody is a synthesized peptide derived from human PAH. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28407/1399_C5_1_selected.jpg
Normal Tissue
Figure 2 High expression in liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_9_4.jpg
Normal Tissue
Figure 3 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_5_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171759-PAH
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Specifications
- Immunogen
- A synthesized peptide derived from human PAH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PAH
- Species Reactivity
- Human
- Clone
- 17D10
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- PAH; PH; PKU; PKU1
- Gene ID
- 5053
- UniProt ID
- P00439
- Long Name
- Phenylalanine hydroxylase
- Sequence Similarities
- Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.
- Protein Refseq
- NP_000268.1
- Function
- Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PAH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1959z | Mouse Anti-PAH Recombinant Antibody (clone 36C11) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| VS3-QX848 | Mouse Anti-PAH Recombinant Antibody (clone 717CT21.1.1) | WB, IHC | Mouse IgG1, κ |
| VS3-CJ204 | Mouse Anti-PAH Recombinant Antibody (VS3-CJ204) | WB, IHC-P | Mouse IgG1, κ |
| VS3-FY1613 | Rabbit Anti-PAH Recombinant Antibody (clone R06-3H4) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2580 | Hi-Affi™ Recombinant Rabbit Anti-PAH Monoclonal Antibody (DS2580AB) | WB, IHC-P, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5160 | Anti-PAH Immunohistochemistry Kit | IHC | |
| VS-0525-XY5161 | Anti-Mouse PAH Immunohistochemistry Kit | IHC | |
| VS-0525-XY5162 | Anti-Rat PAH Immunohistochemistry Kit | IHC |
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