Recombinant Rabbit Anti-PAH Antibody (clone 17D10) (CAT#: VS4-CJ66)
This rabbit anti-PAH antibody (clone 17D10) is a product that recognizes human PAH. The immunogen of this antibody is a synthesized peptide derived from human PAH. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28407/1399_C5_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28407/1399_C5_1_selected.jpg
Figure 2 High expression in liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_9_4.jpg
Figure 3 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_5_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_5_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31642/150831_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171759-PAH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171759-PAH
Specifications
- Immunogen
- A synthesized peptide derived from human PAH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PAH
- Species Reactivity
- Human
- Clone
- 17D10
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- PAH; PH; PKU; PKU1
- Gene ID
- 5053
- UniProt ID
- P00439
- Long Name
- Phenylalanine hydroxylase
- Sequence Similarities
- Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.
- Protein Refseq
- NP_000268.1
- Function
- Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1959z | Mouse Anti-PAH Recombinant Antibody (clone 36C11) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| VS3-QX848 | Mouse Anti-PAH Recombinant Antibody (clone 717CT21.1.1) | WB, IHC | Mouse IgG1, κ |
| VS3-CJ204 | Mouse Anti-PAH Recombinant Antibody (VS3-CJ204) | WB, IHC-P | Mouse IgG1, κ |
| VS3-FY1613 | Rabbit Anti-PAH Recombinant Antibody (clone R06-3H4) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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