Recombinant Rabbit Anti-PARP1 Antibody (clone R05-8E8) (CAT#: VS3-FY1097)
This product is a recombinant rabbit antibody that recognizes PARP1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-8E8 is specific for human, mouse, rat PARP1. The antigen is synthetic peptide of human parp1.
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WB
Figure 1. Recombinant Rabbit Anti-PARP1 Antibody (clone R05-8E8) in WB.
Western Blot analysis of PARP1 in K562, 3T3, Hela lysates using PARP1 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PARP1 Antibody (clone R05-8E8) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using an antibody to PARP1. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PARP1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PARP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-8E8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 113 kDa; Observed MW: 116,89 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Poly(ADP-Ribose) Polymerase 1; ADP-Ribosyltransferase (NAD+; Poly (ADP-Ribose) Polymerase); ADP-Ribosyltransferase Diphtheria Toxin-Like 1; Poly (ADP-Ribose) Polymerase Family, Member 1; NAD(+) ADP-Ribosyltransferase 1; Poly[ADP-Ribose] Synthase 1; EC 2.4.2.30; ADPRT 1; PARP-1; ADPRT; ARTD1
- Gene ID
- 142
- UniProt ID
- P09874
- Cellular Localization
- Nucleus, Chromosome, Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1732z | Mouse Anti-PARP1 Recombinant Antibody (clone 23D11) | WB, ELISA, IHC, IP | Mouse IgG1 |
| MRO-2107-CN | Rabbit Anti-PARP1 Polyclonal Antibody (MRO-2107-CN) | WB, IF, FC | Rabbit IgG |
| MOB-0171F | Mouse Anti-PARP1 Recombinant Antibody (MOB-0171F) | WB | Mouse IgG |
| VS3-CJ544 | Mouse Anti-PARP1 Recombinant Antibody (VS3-CJ544) | WB, IHC | Mouse IgG1 |
| VS3-CJ545 | Rabbit Anti-PARP1 Recombinant Antibody (VS3-CJ545) | WB, ICC, IF, IHC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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