Recombinant Human Anti-PROM1 Antibody (clone 5F5)

CAT#: VS4-CJ93

This human anti-PROM1 antibody (clone 5F5) is a product that recognizes human PROM1. The immunogen of this antibody is recombinant human PROM1 protein. The mAb has been tested for use in ELISA.

Gene Expression
Figure 1 Colon Figure 2 Liver Figure 3 Pancreas Figure 4 Kidney Figure 5 RNA cell line category: Cell line enhanced (AF22, CACO-2, CAPAN-2, EFO-21, NTERA-2, RPTEC TERT1)

Specifications

  • Immunogen
  • Recombinant Human PROM1 protein
  • Host Species
  • Human
  • Type
  • Human IgG1
  • Specificity
  • Human PROM1
  • Species Reactivity
  • Human
  • Clone
  • 5F5
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 0.01M PBS, pH 7.4.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA.

Target

  • Alternative Names
  • RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
  • Long Name
  • Prominin 1
  • Sequence Similarities
  • Belongs to the prominin family.
  • Cellular Localization
  • Cell membrane, Cell projection, Cilium, Endoplasmic reticulum, Membrane
  • Post Translation Modifications
  • Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Function
  • May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617).
    Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

Secondary Antibody

Recommended Dilution Buffer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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