Recombinant Human Anti-PROM1 Antibody (clone 5F5) (CAT#: VS4-CJ93)

• Immunogen
• Recombinant Human PROM1 protein

• Host Species
• Human

• Type
• Human IgG1

• Specificity
• Human PROM1

• Species Reactivity
• Human

• Clone
• 5F5

• Applications
• ELISA

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.01M PBS, pH 7.4.

• Preservative
• 0.03% Proclin 300

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA.

• Alternative Names
• RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

• Gene ID
• 8842

• UniProt ID
• O43490

• Long Name
• Prominin 1

• Sequence Similarities
• Belongs to the prominin family.

• Cellular Localization
• Cell membrane, Cell projection, Cilium, Endoplasmic reticulum, Membrane

• Post Translation Modifications
• Isoform 1 and isoform 2 are glycosylated.
  Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

• Protein Refseq
• NP_001139319.1; NP_001139320.1; NP_001139321.1

• Function
• May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617).
  Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Isotype Control

• MRO-A1587-YJ
• Human IgG Isotype control, Polyclonal

Secondary Antibody

• 2RD-A1571-YJ
• Rabbit Anti-Human IgG1 Secondary antibody (Clone RM117)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS