Recombinant Rabbit Anti-PTPN1 Antibody (clone R05-3B9)
CAT#: VS3-FY1224
This product is a recombinant rabbit antibody that recognizes PTPN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-3B9 is specific for human PTPN1. The antigen is synthetic peptide of human ptp1b.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human PTP1B.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- R05-3B9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- PTPN1; PTP1B
- UniProt ID
- P18031
- Sequence Similarities
- Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
- Protein Refseq
- NP_001265547.1; NP_002818.1
- Function
- Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTPN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0455MZ | Chicken Anti-PTPN1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2928 | Hi-Affi™ Recombinant Rabbit Anti-PTPN1 Monoclonal Antibody (DS2928AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0374-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| HPAB-0375-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0375-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| PSBC-544 | Chicken Anti-PTPN1 Recombinant Antibody scFv Fragment (PSBC-544) | ELISA | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0374-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| HPAB-0375-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0375-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| PFBC-544 | Chicken Anti-PTPN1 Recombinant Antibody Fab Fragment (PFBC-544) | ELISA | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2145-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2145-CN) | WB, IF, IHC | Rabbit IgG |
| MRO-2146-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2146-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ733 | Rabbit Anti-PTPN1 Recombinant Antibody (VS3-CJ733) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-XY1321 | Mouse Anti-PTPN1 Recombinant Antibody (clone 4F8F11) | IHC, FC | Mouse IgG1 |
| VS3-XY1323 | Mouse Anti-PTPN1 Recombinant Antibody (clone 2C11B12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC842 | AbPlus™ Anti-PTPN1 Magnetic Beads (VS-0724-YC842) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC420 | Recombinant Anti-PTPN1 Vesicular Antibody, EV Displayed (VS-0425-YC420) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5858 | Anti-PTPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5859 | Anti-Rat PTPN1 Immunohistochemistry Kit | IHC |
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