Recombinant Rabbit Anti-PTPN1 Antibody (clone R05-3B9) (CAT#: VS3-FY1224)
This product is a recombinant rabbit antibody that recognizes PTPN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-3B9 is specific for human PTPN1. The antigen is synthetic peptide of human ptp1b.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
Specifications
- Immunogen
- Synthetic peptide of human PTP1B.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- R05-3B9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- PTPN1; PTP1B
- UniProt ID
- P18031
- Sequence Similarities
- Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
- Protein Refseq
- NP_001265547.1; NP_002818.1
- Function
- Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0801CT | Recombinant Mouse anti-Human PTPN1 Monoclonal antibody (208BU700) | ELISA, WB | |
| PABC-544 | Chicken Anti-PTPN1 Recombinant Antibody (PABC-544) | ELISA, Inhib | Chicken IgY |
| VS3-QX939 | Mouse Anti-PTPN1 Recombinant Antibody (clone 3C8-H4-A0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK1984 | Rabbit Anti-PTPN1 (pS352) Recombinant Antibody (clone R04-1F2) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK2040 | Rabbit Anti-PTPN1 (pS378) Recombinant Antibody (clone R08-5F7) | WB, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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