Recombinant Rabbit Anti-UBE3A Antibody (clone R02-1A0)
CAT#: VS3-FY1516
This product is a recombinant rabbit antibody that recognizes UBE3A. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-1A0 is specific for human, mouse, rat UBE3A. The antigen is human ube3a recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39410/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular layer.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39410/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9723/82580_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9723/82580_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9723/82580_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9723/82580_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9723/82580_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114062-UBE3A
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ICC
Figure 1. Recombinant Rabbit Anti-UBE3A Antibody (clone R02-1A0) in ICC.
Immunocytochemical analysis of Ubiquitin Ligase E3A (green) in Hela using Ubiquitin Ligase E3A Antibody and DAPI (blue).
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Specifications
- Immunogen
- Human UBE3A recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat UBE3A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1A0
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 101 kDa; Observed MW: 101 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ubiquitin Protein Ligase E3A; Human Papilloma Virus E6-Associated Protein; Human Papillomavirus E6-Associated Protein; Oncogenic Protein-Associated Protein E6-AP; HECT-Type Ubiquitin Transferase E3A; Renal Carcinoma Antigen NY-REN-54; E6AP Ubiquitin-Protein Ligase; EPVE6AP; HPVE6A
- Gene ID
- 7337
- UniProt ID
- Q05086
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UBE3A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2450z | Mouse Anti-UBE3A Recombinant Antibody (clone 44A11) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-2127CT | Recombinant Mouse anti-Human UBE3A Monoclonal antibody (EML2310) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3764 | Rabbit Anti-UBE3A Recombinant Antibody (clone DS3764AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2420 | Anti-UBE3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY7705 | Anti-Human UBE3A Immunohistochemistry Kit | IHC |
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