10-FnIII scaffold protein anti-Chicken Lysozyme (CAT#: SFN-L013)
The tenth fibronectin type III domain (10Fn3) that bound lysozyme was isolated from the library using mRNA Display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=0.35 nM) for lysozyme as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
Figure 2 IHC staining of human salivary gland
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
Figure 3 IHC staining of human skeletal muscle
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
Figure 4 Colon
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- Lysozyme
- Species Reactivity
- Chicken
- Expression Host
- E. coli
- Affinity Constant
- 0.35 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3387z | Mouse Anti-LYZ Recombinant Antibody (clone 39H8) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
| PABL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10) | WB, IHC, FuncS | Human IgG |
| PABL-268 | Mouse Anti-LYZ Recombinant Antibody (PABL-268) | WB, ELISA, FuncS | Mouse IgG |
| MOB-174CQ | Mouse Anti-Lysozyme Antibody (HYHEL-10) | ELISA | Mouse IgG1, κ |
| FAMAB-0132-CN | Recombinant Humanized Anti-LYZ Antibody (HuD1.3) | ELISA, IF | Humanized IgG2a, Κ |
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