What is this antibody used for?

This antibody is used for research purposes to study lysozyme, an important enzyme in innate immunity. It can be applied in experiments like Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), and other functional studies, helping scientists understand its role in immune responses.

Can this product be used for clinical applications?

No, this antibody is strictly for research use only and is not intended for any clinical applications. Its purpose is to advance scientific research and understanding of lysozyme-related functions and diseases.

Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys)

CAT#: PABX-073-F (E)

Anti-Lysozyme antibody is a human Fab class that binds to an Lysozyme. The antibody was generated by immunizing with HEL protein.

Published Data Gene Expression

Figure 1 Blocking isotherms for Chi9.3, Hu9.3, and Hy9.3 Fabs. Hu-Lys Fab was used as a negative control.

Inhibition of binding of CD28-Ig to B7-CHO cells was measured by flow cytometry using varying concentrations of Fabs. The values shown represent the result of one experiment
of three with similar results. The lines represent theoretical simulations of binding based on the final parameters obtained by least-squares.

Tan, P., Mitchell, D. A., Buss, T. N., Holmes, M. A., Anasetti, C., & Foote, J. (2002). “Superhumanized” antibodies: reduction of immunogenic potential by complementarity-determining region grafting with human germline sequences: application to an anti-CD28. The Journal of Immunology, 169(2), 1119-1125.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human salivary gland

High expression. RNA expression: 21115.5 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg

Normal Tissue Figure 3 IHC staining of human skeletal muscle

Low expression. RNA expression: 34.0 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enriched (OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ

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Specifications

Immunogen
HEL

Host Species
Human

Derivation
Human

Type
Fab

Antibody Isotype
IgG

Specificity
Tested positive against native Hen Lysozyme

Species Reactivity
Hen

Clone
HuLys

Applications
WB 1:1000-1:5000
ELISA 1:10000-1:20000
FuncS (assay dependent)

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
Assay-depended

Target

Alternative Names
LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C

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Breast cancer biomarkers at key points during disease progression

Great Research Tool

This antibody is an excellent tool for my lab's research. Its high specificity has allowed us to accurately quantify lysozyme levels in our assays. Having a consistent batch-to-batch performance is crucial for our experiments, and this product delivers that reliability.

Reliable for Immunoassays

We've been using this product for both WB and ELISA with great results. Its purity and formulation make it particularly effective in delivering consistent bands in Western blots, a key factor in our experimental validations.

Customized Solutions Available

I was pleased to find that Creative Biolabs could provide customized conjugations for our research needs. This flexibility allowed us to optimize our experiments and improve our workflow without compromising on the antibody's performance.

Q&As

What is this antibody used for?

A: This antibody is used for research purposes to study lysozyme, an important enzyme in innate immunity. It can be applied in experiments like Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), and other functional studies, helping scientists understand its role in immune responses.
Can this product be used for clinical applications?

A: No, this antibody is strictly for research use only and is not intended for any clinical applications. Its purpose is to advance scientific research and understanding of lysozyme-related functions and diseases.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-073-F (E).pdf

MSDS

MSDS - PABX-073-F (E).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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