Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys)
CAT#: PABX-073-F (E)
Anti-Lysozyme antibody is a human Fab class that binds to an Lysozyme. The antibody was generated by immunizing with HEL protein.
Published Data
Gene Expression
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Figure 1 Blocking isotherms for Chi9.3, Hu9.3, and Hy9.3 Fabs. Hu-Lys Fab was used as a negative control.
Inhibition of binding of CD28-Ig to B7-CHO cells was measured by flow cytometry using varying concentrations of Fabs. The values shown represent the result of one experiment
of three with similar results. The lines represent theoretical simulations of binding based on the final parameters obtained by least-squares.
Tan, P., Mitchell, D. A., Buss, T. N., Holmes, M. A., Anasetti, C., & Foote, J. (2002). “Superhumanized” antibodies: reduction of immunogenic potential by complementarity-determining region grafting with human germline sequences: application to an anti-CD28. The Journal of Immunology, 169(2), 1119-1125.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
High expression. RNA expression: 21115.5 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human skeletal muscle
Low expression. RNA expression: 34.0 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
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Specifications
- Immunogen
- HEL
- Host Species
- Human
- Derivation
- Human
- Type
- Fab
- Specificity
- Tested positive against native Hen Lysozyme
- Species Reactivity
- Hen
- Clone
- HuLys
- Applications
- WB 1:1000-1:5000
ELISA 1:10000-1:20000
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C
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There are currently no Customer reviews or questions for PABX-073-F (E). Click the button above to contact us or submit your feedback about this product.
Great Research Tool
This antibody is an excellent tool for my lab's research. Its high specificity has allowed us to accurately quantify lysozyme levels in our assays. Having a consistent batch-to-batch performance is crucial for our experiments, and this product delivers that reliability.
Reliable for Immunoassays
We've been using this product for both WB and ELISA with great results. Its purity and formulation make it particularly effective in delivering consistent bands in Western blots, a key factor in our experimental validations.
Customized Solutions Available
I was pleased to find that Creative Biolabs could provide customized conjugations for our research needs. This flexibility allowed us to optimize our experiments and improve our workflow without compromising on the antibody's performance.
Q&As
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What is this antibody used for?
A: This antibody is used for research purposes to study lysozyme, an important enzyme in innate immunity. It can be applied in experiments like Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), and other functional studies, helping scientists understand its role in immune responses.
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Can this product be used for clinical applications?
A: No, this antibody is strictly for research use only and is not intended for any clinical applications. Its purpose is to advance scientific research and understanding of lysozyme-related functions and diseases.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
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| PFBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); Fab Fragment | WB, FuncS | Mouse Fab |
| PFBZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1); Fab Fragment | WB, IF, FuncS | Mouse Fab |
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| CAT | Product Name | Application | Type |
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| PSBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); scFv Fragment | WB, FuncS | Mouse scFv |
| PSBZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4); scFv Fragment | ELISA | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
| CAT | Product Name | Application | Type |
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| PABX-074 | Recombinant Mouse Anti-Lysozyme Antibody (PABX-074) | WB, ELISA, FuncS | IgG |
| PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
| PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
| PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (PABX-074-S (P)) | WB, ELISA, FuncS | scFv |
| PABX-139-F (E) | Mouse Anti-LYZ Recombinant Antibody; Fab Fragment (PABX-139-F (E)) | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
| PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
| PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0525-XY4146 | Anti-LYZ Immunohistochemistry Kit | IHC | |
| VS-0525-XY4147 | Anti-Mouse LYZ Immunohistochemistry Kit | IHC |
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