Ankyrin repeat scaffold protein anti-Human IL4 (CAT#: SAR-L261)
The Ankyrin repeat that bound IL4 was isolated from the library using ribosome display techonology. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops, first discovered in signaling proteins in yeast Cdc10 and Drosophila Notch. Domains consisting of ankyrin repeats mediate protein–protein interactions and are among the most common structural motifs in known proteins. After affinity maturation, Ankyrin repeat variants were identified having affinities (KD=9.0 pM) for IL4 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
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Normal Tissue
RNA Expression
Figure 1 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg
Figure 2 RNA cell line category: Cell line enriched (MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113520-IL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113520-IL4
Specifications
- Scaffold Name
- Ankyrin repeat
- Origin
- Ankyrin repeat proteins
- Core Structure
- 2α-helices, β-turn
- Variable Regions
- b-turn,1a-helix and loop
- Target
- IL4
- Species Reactivity
- Human
- Expression Host
- BL21 (DE3) cells
- Affinity Constant
- 9.0 pM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
Related Resources
Asthma
Autoimmune Thyroid Disease
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-102 | Mouse Anti-Il4 Recombinant Antibody (clone 11B11) | ELISA, FC | Mouse IgG |
| MOB-085LC | Mouse Anti-IL4 Recombinant Antibody (clone YC133) | ELISA, FuncS | Mouse IgG |
| MRO-0832-CN | Rabbit Anti-IL4 Recombinant Antibody (clone JA86-10) | WB, IF, IHC | Rabbit IgG |
| MRO-1968-CN | Rabbit Anti-IL4 Polyclonal Antibody (MRO-1968-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0746F | Rat Anti-IL4 Antibody (clone AR.98) | FC, IF | Rat IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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