Anti-Human IL4 Recombinant Antibody (TAB-221) (CAT#: TAB-221)

Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA

ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB

Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB

Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control