Recombinant Mouse Anti-αVβ3 Ectodomain Antibody scFv Fragment (17E6) (CAT#: PABX-191-S (P))

Figure 1 17E6 perturbs M21 adhesion to vitronectin but not to other matrix components.

The inhibitory effect of 17E6 (αv, closed circles) and P4C10 (β1, open circles) on M21 cell attachment to vitronectin (A), laminin (B) or collagen type I (C) was studied. 17E6 only blocked cell attachment on vitronectin-coated surfaces. Whilst P4C10 only blocked cell attachment on collagen type I and laminin.

Mitjans, F., Sander, D., Adán, J., Sutter, A., Martinez, J. M., Jaggle, C. S., ... & Goodman, S. L. (1995). An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. Journal of cell science, 108(8), 2825-2838.

Figure 2 Species-dependent inhibition of cell attachment by 17E6 and species-independent inhibition by cilengitide.

Representative cell adhesion inhibition assay comparing human melanoma cell line WM164 (circles), monkey cell line CV-1 (triangles), and rat cell line Rat-1 (boxes). Filled symbols show response to 17E6, and open symbols show response to the RGD-peptide cilengitide. Human and monkey cells are inhibited, and rat cells are not affected by 17E6. Each species is inhibited by cilengitide.

Mahalingam, B., Van Agthoven, J. F., Xiong, J. P., Alonso, J. L., Adair, B. D., Rui, X., ... & Goodman, S. L. (2014). Atomic basis for the species-specific inhibition of αV integrins by mAb 17E6 is revealed by the crystal structure of αVβ3 ectodomain-17E6 Fab complex. Journal of Biological Chemistry, jbc-M113.