Recombinant Mouse Antibody ( 17E6) is capable of binding to ITGAV.
Figure 2 Integrin alpha V in Human melanoma cells by Flow Cytometery.
Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
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For Research Use Only. Not For Clinical Use.