Mouse Anti-ITGAV Recombinant Antibody (clone 17E6)

Figure 1 Integrin alpha V in Human prostate cells by Immunocytochemistry/Immunofluorescence.

Figure 2 Integrin alpha V in Human melanoma cells by Flow Cytometery.

Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.