Recombinant Mouse Anti-AAV VP1 Antibody (A20) (CAT#: PABL-390)

Recombinant Mouse Antibody (A20) is capable of binding to AAV VP1 , expressed in Chinese Hamster Ovary cells (CHO). A20 is a monoclonal antibody which neutralizes AAV-2 and AAV-3B subsequent to primary receptor binding.

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ICC

Figure 1 Time course of AAV infection using A20 immunocytochemistry.

HeLa cells were infected with wild-type AAV at a MOI of 10,000 particles per cell for 10 min at 37°C, washed to remove unadsorbed virus, and incubated at 37°C. Viral particles were detected with A20 monoclonal antibody.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.

IF

Figure 2 Time course of AAV infection in the presence of Ad coinfection.

HeLa cells were infected with wild-typeAAV as in panel A and incubated at 37°C for 10 min. Samples were then stained with A20 antibody, which stains nucleic acid in the nucleus.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.


Specifications

  • Immunogen
  • AAV Capsid protein VP1
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native AAV VP1
  • Species Reactivity
  • AAV
  • Clone
  • A20
  • Applications
  • ICC, IF

Applications

  • Application Notes
  • This antibody has been reported for use in Immunocytochemistry and Immunofluorescence.

Target

  • Alternative Names
  • Capsid protein VP1; vp1

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "VP1"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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