Recombinant Mouse Anti-AAV VP1 Antibody (A20)

Figure 1 Time course of AAV infection using A20 immunocytochemistry.

HeLa cells were infected with wild-type AAV at a MOI of 10,000 particles per cell for 10 min at 37°C, washed to remove unadsorbed virus, and incubated at 37°C. Viral particles were detected with A20 monoclonal antibody.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.

Figure 2 Time course of AAV infection in the presence of Ad coinfection.

HeLa cells were infected with wild-typeAAV as in panel A and incubated at 37°C for 10 min. Samples were then stained with A20 antibody, which stains nucleic acid in the nucleus.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.