Recombinant Mouse Anti-AAV VP1 Antibody (A20) (CAT#: PABL-390)

Figure 1 Time course of AAV infection using A20 immunocytochemistry.

HeLa cells were infected with wild-type AAV at a MOI of 10,000 particles per cell for 10 min at 37°C, washed to remove unadsorbed virus, and incubated at 37°C. Viral particles were detected with A20 monoclonal antibody.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.

Figure 2 Time course of AAV infection in the presence of Ad coinfection.

HeLa cells were infected with wild-typeAAV as in panel A and incubated at 37°C for 10 min. Samples were then stained with A20 antibody, which stains nucleic acid in the nucleus.

Xiao, W., Warrington, K. H., Hearing, P., Hughes, J., & Muzyczka, N. (2002). Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2. Journal of virology, 76(22), 11505-11517.

Figure 1 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in SDS-PAGE

SDS-PAGE analysis of PABL-390 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively.

Figure 2 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in SEC-HPLC

The purity of PABL-390 was 99.7796% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in DB

Dot Blot analysis of PABL-390 was performed by coating with AAV2/2 virus particles (6.78E+12).

PABL-390 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 4 Mouse Anti-AAV VP1 Recombinant Antibody (PABL-390) in ELISA

ELISA analysis of PABL-390 was performed by coating with AAV2/2 virus particles (6.78E+12). Then blocked with BSA and incubated with PABL-390. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.