Human Anti-ALK Recombinant Antibody (clone ESBA521) (CAT#: NS-004CN)
Anti-ALK antibody is an antibody specific for human ALK, which is suitable for the local treatment of tumors or cancer, in particular glioblastoma.
Specific Inquiry
ELISA
Figure 1 ELISA experiment wherein the binding characteristics of improved scFvs are compared to that of the framework they originate from.
ELISA
Figure 2 ELISA experiment comparing the ESBA512 to the improved tertiary binders.
FuncS
Figure 3 The effects of different concentrations of His-tagged ESBA521, scFv-14-His and scFv-FW-His on cell proliferation in vitro of SW13 cells stably expressing PTN in the absence of serum.
His-tagged ESBA521: ESBA521 is referred to as scFv-107, circles; scFv-14-His: scFv-14 has an affinity to ALK in the micromolar range, triangles; scFv-FW-His: a unspecific scFv_control having the identical framework 4.4 as ESBA521 or scFv-14, but different CDRs, squares. One representative of three independent experiments with mean + S. E. of triplicate wells is shown.
WB
Figure 4 Western blot evidencing the tet-inducible expression of FLAG-scFv framework control and FLAG-scFv-107 (FLAG-ESBA521) from the stably trans-fected U87MG cell lines.
24 hours post-plating the U87MG cells stably transfected TRex-DEST-30-FLAG-scFv-FW or T-Rex-DEST-30-FLAG-scFv-107 with cells were induced with 1 mg/ml of tetracycline for 24 hours. The presence of FLAG-scFv-FW and FLAG-scFv-107 in conditioned media from uninduced (-) and induced (+) cell lines was detected by immunoblotting with anti-FLAG HRP.
Specifications
- Immunogen
- Human ALK Polypeptide
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- ESBA521
- Applications
- ELISA, WB, FuncS
- Related Disease
- Metastatic cancers or tumors
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Functional Assay.
Target
- Alternative Names
- Anaplastic Lymphoma Receptor Tyrosine Kinase; CD246 Antigen; EC 2.7.10.1; Anaplastic Lymphoma Kinase (Ki-1); Mutant Anaplastic Lymphoma Kinase; Anaplastic Lymphoma Kinase; NBLST3; CD246
- Gene ID
- 238
- UniProt ID
- Q9UM73
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone ESBA521"
See other products for "ALK"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0296MZ | Mouse Anti-ALK Recombinant Antibody (clone BLK2) | IHC | Mouse IgG3 |
| HPAB-0138-YC | Mouse Anti-ALK Recombinant Antibody (HPAB-0138-YC) | ELISA, FC, WB, Inhib | Mouse IgG |
| HPAB-0139-YC | Mouse Anti-ALK Recombinant Antibody (HPAB-0139-YC) | ELISA, WB, FC, Agonist | Mouse IgG |
| HPAB-0140-YC | Mouse Anti-ALK Recombinant Antibody (HPAB-0140-YC) | ELISA, FC, WB | Mouse IgG |
| HPAB-0141-YC | Mouse Anti-ALK Recombinant Antibody (HPAB-0141-YC) | ELISA, FC, RIA, Inhib | Mouse IgG, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0147 | Hi-Affi™ Rabbit Anti-ALK Recombinant Antibody (clone DS147AB) | IHC-P | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0138-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0138-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0139-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0139-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0140-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0140-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0141-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0141-YC-S(P)) | ELISA, FC, RIA | Mouse scFv |
| HPAB-0143-YC-S(P) | Mouse Anti-ALK Recombinant Antibody (clone D10); scFv Fragment | ELISA, FC, Stim | Mouse scFv |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-004CN-F(E) | Human Anti-ALK Recombinant Antibody (clone ESBA521); Fab Fragment | ELISA, WB | Human Fab |
| NS-004CN-S(P) | Human Anti-ALK Recombinant Antibody (clone ESBA521); scFv Fragment | ELISA, WB | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1817LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1817LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1818LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1818LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1819LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1819LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1820LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1820LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1821LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1821LY-F(E)) | ELISA, WB, FC | Mouse Fab |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF574 | A*02:01/Human Mutant anaplastic lymphoma kinase (SLAMLDLLHV) MHC Pentamer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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