Hi-Affi™ Rabbit Anti-ASPH Recombinant Antibody (clone DS269AB)
CAT#: MOR-0269
Recombinant Rabbit anti-ASPH monoclonal antibody for WB, IHC-P, ICC/IF, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55161/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/1873_A7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/133131_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/133131_A_7_3.jpg
Normal Tissue
Figure 6 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/133131_A_2_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/133131_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59303/133131_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198363-ASPH
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS269AB
- Applications
- WB, IHC-P, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Citations
-
Babich, Michael, et al. "Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen." Heliyon 8.3 (2022). https://doi.org/10.1016/j.heliyon.2022.e08988This article distinguishes labyrinthin as a unique tumor-specific antigen (TSA) exclusively expressed on the surface of adenocarcinoma cells, differentiating it from the related intracellular protein aspartyl/asparaginyl β-hydroxylase (ASPH). Through immunohistochemistry, FACS analysis, confocal microscopy, and cell transfection studies, the researchers demonstrate that labyrinthin not only serves as a marker but appears functional in the cancer phenotype, with its expression promoting cancerous characteristics in normal cells and its inhibition normalizing cancer cell morphology.
Creative Biolabs provided recombinant human IgG1 (CAT#: MHH-78-F(E)) antibodies that bind to ASPH in HEK 293 cells as well as polyclonal antibodies (CAT#: MOR-0269) targeting ASPH amino acids 250-350. These antibodies helped the researchers distinguish between ASPH and labyrinthin binding patterns, clarifying the cellular localization and expression patterns of these proteins and establishing labyrinthin as a distinct cell-surface target for potential adenocarcinoma immunotherapy approaches.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ASPH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-78 | Recombinant Anti-human ASPH Antibody | WB, FC, FuncS | IgG |
| MHH-78 | Recombinant Human Anti-human ASPH Antibody | IF, FACS, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-78-F(E) | Recombinant Anti-human ASPH Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-78-F(E) | Recombinant Human Anti-human ASPH Antibody Fab Fragment | WB, IHC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-78-S(P) | Recombinant Anti-human ASPH Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| MHH-78-S(P) | Recombinant Human Anti-human ASPH Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY189 | Anti-ASPH Immunohistochemistry Kit | IHC | |
| VS-0525-XY564 | Anti-Human ASPH Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY12 | Human Anti-ASPH scFv-Fc Chimera (VS-0625-FY12) | FC | Human IgG1, scFv-Fc |
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