Human Anti-CD3 Recombinant Antibody; Fab Fragment (TAB-118CL) (CAT#: TAB-118CL)

• Comprehensive datasheet
  This antibody comes with an extremely extensive datasheet. It had all the information needed to begin going, and the suggested methods operated flawlessly. beneficial to both novice and seasoned users.
• Long shelf life
  For any lab, this is convenient because the antibody has an extended shelf life when maintained properly. We have been using it for a long time, and it has never lost its effectiveness.
• Minimal non-specific binding
  The limited non-specific binding of this antibody is one of its finest qualities. The results are clear and accurate in both immunofluorescence and Western blotting, which is essential for exact data interpretation.

1. Q 1: How should I dilute the antibody for my application?

   A: Starting dilutions can change based on your application. A 1:1,000 dilution is advised for Western blotting, but a 1:100 dilution can be suitable for immunofluorescence or immunohistochemistry. Always adjust dilutions to meet the needs of the experiment.

2. Q 2: What controls should I include in my experiment?

   A: To confirm the antibody's performance and specificity, always include both positive and negative controls in your tests. Utilize known to express the target protein tissues or cell lines, for instance, as a positive control.

3. Q 3: How can I check the specificity of the antibody?

   A: A knockdown or knockout experiment of the target protein in your sample and a comparison of the signal to untreated controls can be used to verify specificity. Confidence can also be derived from the manufacturer's validation using other assays.

View the frequently asked questions answered by Creative Biolabs Support.