Anti-Human CD3 Recombinant Antibody (OKT3) (CAT#: TAB-0416CL)

This anti-CD3 monoclonal antibody can be used in the treatment and optionally also prevention of diseases and disorders, such as autoimmune disorders, infectious diseases, and transplant rejection, that are susceptible to amelioration by binding to CD3.


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  • Published Data
  • Tested Data
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FC

Figure 1 Soluble anti-CD3 mAbs induce death of preactivated and cycling human peripheral T cells.

Figure 1 Soluble anti-CD3 mAbs induce death of preactivated and cycling human peripheral T cells.

T cells were preactivated for 24 h (left panel) or 72 h (right panel) by solid phase-bound anti-CD3 mAb, BC3. T cells were harvested, one aliquot was used for cell cycle analysis, and other aliquots were exposed for 24 h to 0.2 μg/ml of the anti-CD3 mAbs, HuM291-IgG2M3 or OKT3, or to a murine IgG2a mAb of irrelevant specificity in the presence of 50 IU/ml IL-2 in CM containing 10% normal human serum.

Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X. Z., & Anasetti, C. (2000). Non-Fc receptor-binding humanized anti-CD3 antibodies induce apoptosis of activated human T cells. The Journal of Immunology, 165(11), 6205-6213.

IB

Figure 2 HuM291 induces greater and more sustained ERK phosphorylation in activated human T cells than OKT3.

Figure 2 HuM291 induces greater and more sustained ERK phosphorylation in activated human T cells than OKT3.

Cells were cultured in medium alone or were stimulated at 37°C with 50 μg/μl HuM291 or 50 μg/μl OKT3 for the indicated time in minutes. Activated cells stimulated with PMA (15 ng/ml) plus ionomycin (700 ng/ml) for 10 min were analyzed as a positive control for ERK phosphorylation. Cells receiving 50 μg/μl anti-human IgG2 or 50 μg/μl anti-mouse IgG2a for 10 min were analyzed as additional negative controls for ERK phosphorylation. Whole-cell lysates were prepared, and 20 μg of protein was separated by SDS-PAGE as described in Materials and Methods. ERK phosphorylation (p-ERK1 and p-ERK2) was determined by Western blotting with an anti-phospho-ERK mAb (top panel). Subsequently, the same nitrocellulose membrane was stripped and reprobed with an anti-ERK2 mAb to determine ERK2 expression for each time interval and condition (bottom panel). A representative experiment of three performed is shown.

Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X. Z., & Anasetti, C. (2000). Non-Fc receptor-binding humanized anti-CD3 antibodies induce apoptosis of activated human T cells. The Journal of Immunology, 165(11), 6205-6213.

ELISA

Figure 3 Anti-CD3 mAb variants induce more T cell death and release of IFN-γ than do murine anti-CD3 mAbs OKT3 or M291.

Figure 3 Anti-CD3 mAb variants induce more T cell death and release of IFN-γ than do murine anti-CD3 mAbs OKT3 or M291.

Supernatants from samples cultured in parallel to those for the cell death assays, but without exogenous IL-2, and with CM containing FCS instead of pooled human serum were assayed for the cytokines indicated.

Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X. Z., & Anasetti, C. (2000). Non-Fc receptor-binding humanized anti-CD3 antibodies induce apoptosis of activated human T cells. The Journal of Immunology, 165(11), 6205-6213.


Specifications

  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • OKT3
  • Applications
  • FC, IB, ELISA
  • Related Disease
  • Inflammatory diseases, autoimmune disorders, infectious diseases, and transplant rejection

Applications

  • Application Notes
  • The Cluster of Differentiation 3 (CD3) antibody has been reported in applications of Flow Cytometry, Immunoblotting, Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Chimeric Antibody

CAT Product Name Application Type
TAB-0417CL Chimeric Anti-Human CD3 Recombinant Antibody ELISA, FC, FuncS Chimeric (mouse/human)
TAB-071LC Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-072LC Chimeric Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-071LC-S(P) Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-339CQ Mouse Anti-CD3 Recombinant Antibody (clone SPV-T3b) FC, Block, IHC-Fr, IF, WB Mouse IgG2a
NEUT-340CQ Mouse Anti-CD3 Recombinant Antibody (clone UCHT1) FC, IHC-Fr, IHC-P, ICC, IP, WB, Activ, Block Mouse IgG1, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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