Anti-Mouse CD3 Recombinant Antibody (Dow2)

Figure 1 Recognition of CD3ε on mouse T cells by Dow2.

Suspensions of splenic pan-T cells from normal C57BL/6JJcl donors were used for flow cytometry analyses comparing the binding profiles of T cells incubated with Dow2 to T cells incubated with control anti-mouse CD3ε Abs 17A2 or 145-2C11 (1 μg/ml).

Sugita, S., Shimizu, J., Makabe, K., Keino, H., Watanabe, T., & Takahashi, M. (2017). Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody. Arthritis research & therapy, 19(1), 176.

Figure 2 Evaluation of ocular inflammation in experimental autoimmune uveitis (EAU) mice treated with Dow2 or rat IgG isotype control.

At 21 days postimmunization, the retinas of EAU mice were examined by FA, hematoxylin and eosin (H&E) staining of paraffin-embedded sections, and immunohistochemistry for CD3+ cells. Representative images are shown for immunohistochemistry of retinal sections (scale bar = 20 μm).

Sugita, S., Shimizu, J., Makabe, K., Keino, H., Watanabe, T., & Takahashi, M. (2017). Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody. Arthritis research & therapy, 19(1), 176.