Anti-Mouse CD3 Recombinant Antibody (Dow2) (CAT#: TAB-0365CL)

The anti-CD3 monoclonal antibody specifically target CD3 and can induce downmodulationg of the TCR/CD3 complex in mouse CD4+ T cells without activating T cells. Thus, the specific antbody can be used for suppressing immune responses and inducing immunosupression.

Host: Rat

Applications: FC, IHC


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FC

Figure 1 Recognition of CD3ε on mouse T cells by Dow2.

Suspensions of splenic pan-T cells from normal C57BL/6JJcl donors were used for flow cytometry analyses comparing the binding profiles of T cells incubated with Dow2 to T cells incubated with control anti-mouse CD3ε Abs 17A2 or 145-2C11 (1 μg/ml).

Sugita, S., Shimizu, J., Makabe, K., Keino, H., Watanabe, T., & Takahashi, M. (2017). Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody. Arthritis research & therapy, 19(1), 176.

IHC

Figure 2 Evaluation of ocular inflammation in experimental autoimmune uveitis (EAU) mice treated with Dow2 or rat IgG isotype control.

At 21 days postimmunization, the retinas of EAU mice were examined by FA, hematoxylin and eosin (H&E) staining of paraffin-embedded sections, and immunohistochemistry for CD3+ cells. Representative images are shown for immunohistochemistry of retinal sections (scale bar = 20 μm).

Sugita, S., Shimizu, J., Makabe, K., Keino, H., Watanabe, T., & Takahashi, M. (2017). Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody. Arthritis research & therapy, 19(1), 176.


Specifications

  • Host Species
  • Rat
  • Specificity
  • Mouse
  • Clone
  • Dow2
  • Applications
  • FC, IHC

Applications

  • Application Notes
  • The CD3 antibody has been reported in applications of Flow Cytometry, Immunohistochemistry.

Target

  • Alternative Names
  • CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Humanized Antibody

CAT Product Name Application Type
TAB-118CL Human Anti-CD3 Recombinant Antibody; Fab Fragment (TAB-118CL) ELISA Fab fragment
TAB-0418CL Humanized Anti-Human CD3 Recombinant Antibody ELISA, FC, FuncS Humanized Antibody
TAB-075LC-S(P) Human Anti-CD3 Recombinant Antibody; scFv Fragment (TAB-075LC-S(P)) ELISA Human scFv
TAB-076LC-S(P) Human Anti-CD3 Recombinant Antibody; scFv Fragment (TAB-076LC-S(P)) ELISA Human scFv
TAB-077LC-S(P) Human Anti-CD3 Recombinant Antibody; scFv Fragment (TAB-077LC-S(P)) ELISA Human scFv

Mouse Antibody

CAT Product Name Application Type
TAB-0422CL-S(P) Mouse Anti-CD3 Recombinant Antibody; scFv Fragment (TAB-0422CL-S(P)) ELISA Mouse scFv
TAB-097LC Mouse Anti-CD3 Recombinant Antibody (TAB-097LC) ELISA, SPR Mouse IgG
TAB-099LC Mouse Anti-CD3 Recombinant Antibody (TAB-099LC) ELISA, SPR Mouse IgG
TAB-070LC-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (TAB-070LC-F(E)) ELISA Mouse Fab
TAB-073LC-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (TAB-073LC-F(E)) ELISA Mouse Fab

Chimeric Antibody

CAT Product Name Application Type
TAB-0417CL-F(E) Chimeric Anti-Human CD3 Recombinant Antibody Fab Fragment ELISA, FC, FuncS Chimeric (mouse/human)
TAB-071LC Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-072LC Chimeric Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-071LC-S(P) Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-339CQ Mouse Anti-CD3 Recombinant Antibody (clone SPV-T3b) FC, Block, IHC-Fr, IF, WB Mouse IgG2a
NEUT-340CQ Mouse Anti-CD3 Recombinant Antibody (clone UCHT1) FC, IHC-Fr, IHC-P, ICC, IP, WB, Activ, Block Mouse IgG1, κ

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0053-YC-S(P) Mouse Anti-CD3 Recombinant Antibody (clone G3(3)); scFv Fragment FC, IHC Mouse scFv
HPAB-1909LY-S(P) Human Anti-CD3 Recombinant Antibody; scFv Fragment (HPAB-1909LY-S(P)) ELISA, FC Humanized scfv
HPAB-0441-YJ-S(P) Mouse Anti-CD3 Recombinant Antibody (clone m3E3); scFv Fragment ELISA, FC Mouse scFv
HPAB-0442-YJ-S(P) Mouse Anti-CD3 Recombinant Antibody (clone hc3E3); scFv Fragment ELISA, FC Mouse scFv
HPAB-1181WJ-S(P) Human Anti-CD3 Recombinant Antibody (clone CBLC-007); scFv Fragment ELISA Humanized scFv

Recombinant Antibody

CAT Product Name Application Type
FAMAB-0043CQ Mouse Anti-CD3 Recombinant Antibody (clone 898H2-6-15) ELISA, FuncS Mouse IgG2a, κ
FAMAB-0044CQ Mouse Anti-CD3 Recombinant Antibody (clone FN18) ELISA, WB, FC, FuncS Mouse IgG, κ
FN-205CQ Mouse Anti-CD3 Recombinant Antibody (clone CBL-1337) FC, FuncS, IHC, IF, IP Mouse IgG3, κ
FN-252CQ Mouse Anti-CD3 Recombinant Antibody (clone SK7) FC, FuncS, IF, IHC-Fr, IHC Mouse IgG1, κ
HPAB-0543-WJ Mouse Anti-CD3 Recombinant Antibody (clone YTH 12.5) ELISA, FC Mouse IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0667LY-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (HPAB-0667LY-F(E)) ELISA, FC Mouse Fab
HPAB-0668LY-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (HPAB-0668LY-F(E)) ELISA, FC Mouse Fab
HPAB-0669LY-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (HPAB-0669LY-F(E)) ELISA, FC Mouse Fab
HPAB-0670LY-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (HPAB-0670LY-F(E)) ELISA, FC Mouse Fab
HPAB-0671LY-F(E) Mouse Anti-CD3 Recombinant Antibody; Fab Fragment (HPAB-0671LY-F(E)) ELISA, FC Mouse Fab

Single-domain Antibody

CAT Product Name Application Type
HPAB-M0378-YC Recombinant Llama Anti-CD3 Single Domain Antibody (A30228) ELISA, FC Llama VHH
HPAB-M0379-YC Recombinant Llama Anti-CD3 Single Domain Antibody (A30311) ELISA, FC Llama VHH
HPAB-1029WJ Recombinant Camelid Anti-CD3 Single Domain Antibody ELISA Camelid VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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