Recombinant Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) (CAT#: HPAB-M0378-YC)

Provided is an anit-CD3 single-domain antibody. The anit-CD3 single-domain antibody antagonizes the human CD3 epsilon N-terminal part, and the anit-CD3 single-domain antibody can be used for measurement materials such as CD3 and other functional protein requiring the CD3 such as a bispecific antibody.

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SDS-PAGE

Figure 1 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SDS-PAGE

Figure 1 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-M0378-YC in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced and non-reduced conditions, the purified proteins migrate as about 11-17 kDa that is consistent with theoretical size.

SEC-HPLC

Figure 2 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SEC-HPLC

Figure 2 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SEC-HPLC

The purity of HPAB-M0378-YC was analyzed by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

DB

Figure 3 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in DB

Figure 3 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in DB

Dot blot analysis of HPAB-M0378-YC was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa).

HPAB-M0378-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP mouse anti-His tag

WB

Figure 4 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in WB

Figure 4 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in WB

Western blot analysis of HPAB-M0378-YC was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for one hour. Membranes were probed with HPAB-M0378-YC (2 ng/μL) and HRP mouse anti-His tag as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 5 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in ELISA

Figure 5 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in ELISA

ELISA analysis of HPAB-M0378-YC was performed by coating with human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa). Then blocked with BSA and incubated with HPAB-M0378-YC. The HRP-conjugated mouse anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

FACS

Figure 6 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in FACS

Figure 6 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in FACS

Flow cytometry analysis of Jurkat cells incubated with anti-human CD3 recombinant antibody cat# HPAB-M0378-YC. Jurkat cells were stained with HPAB-M0378-YC (blue histogram, TE232382) or isotype control antibody (yellow histogram), followed by APC-conjugated goat anti-His tag secondary antibody. The used concentration of HPAB-M0378-YC was 10 µg per 5 x 10^6 cells.


Specifications

  • Host Species
  • Llama
  • Type
  • Llama VHH
  • Specificity
  • Human
  • Species Reactivity
  • Human
  • Clone
  • HPAB-M0378-YC
  • Applications
  • ELISA, Flow Cytometry

Target

  • Alternative Names
  • Cluster of differentiation 3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chimeric Antibody

CAT Product Name Application Type
TAB-0417CL Chimeric Anti-Human CD3 Recombinant Antibody ELISA, FC, FuncS Chimeric (mouse/human)
TAB-071LC Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-072LC Chimeric Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-071LC-S(P) Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)

Humanized Antibody

CAT Product Name Application Type
TAB-0418CL Humanized Anti-Human CD3 Recombinant Antibody ELISA, FC, FuncS Humanized Antibody
TAB-075LC Human Anti-CD3 Recombinant Antibody (TAB-075LC) ELISA, FC Humanized antibody
TAB-076LC Human Anti-CD3 Recombinant Antibody (TAB-076LC) ELISA, FC, FuncS Human IgG1, κ
TAB-077LC Human Anti-CD3 Recombinant Antibody (TAB-077LC) ELISA, FC, FuncS Human IgG1, κ
TAB-078LC Human Anti-CD3 Recombinant Antibody (TAB-078LC) ELISA, FC, FuncS Human IgG1, κ

Blocking Antibody

CAT Product Name Application Type
NEUT-339CQ Mouse Anti-CD3 Recombinant Antibody (clone SPV-T3b) FC, Block, IHC-Fr, IF, WB Mouse IgG2a
NEUT-340CQ Mouse Anti-CD3 Recombinant Antibody (clone UCHT1) FC, IHC-Fr, IHC-P, ICC, IP, WB, Activ, Block Mouse IgG1, κ

Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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