Recombinant Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) (CAT#: HPAB-M0378-YC)

Figure 1 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-M0378-YC in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced and non-reduced conditions, the purified proteins migrate as about 11-17 kDa that is consistent with theoretical size.

Figure 2 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in SEC-HPLC

The purity of HPAB-M0378-YC was analyzed by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in DB

Dot blot analysis of HPAB-M0378-YC was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa).

HPAB-M0378-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP mouse anti-His tag

Figure 4 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in WB

Western blot analysis of HPAB-M0378-YC was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for one hour. Membranes were probed with HPAB-M0378-YC (2 ng/μL) and HRP mouse anti-His tag as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

Figure 5 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in ELISA

ELISA analysis of HPAB-M0378-YC was performed by coating with human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa). Then blocked with BSA and incubated with HPAB-M0378-YC. The HRP-conjugated mouse anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 6 Llama Anti-CD3 Single Domain Antibody (HPAB-M0378-YC) in FACS

Flow cytometry analysis of Jurkat cells incubated with anti-human CD3 recombinant antibody cat# HPAB-M0378-YC. Jurkat cells were stained with HPAB-M0378-YC (blue histogram, TE232382) or isotype control antibody (yellow histogram), followed by APC-conjugated goat anti-His tag secondary antibody. The used concentration of HPAB-M0378-YC was 10 µg per 5 x 10^6 cells.