Rat Anti-CD5 Recombinant Antibody (clone 53-7.3) (CAT#: NEUT-392CQ)

Figure 1 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in WB

Western blot analysis of NEUT-392CQ-Fab was performed by loading recombinant mouse CD5 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with purified antibody and HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 2 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in ELISA

ELISA analysis of NEUT-392CQ-Fab was performed by coating with recombinant mouse CD5 protein. Then blocked with BSA and incubated with NEUT-392CQ-Fab. The HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.