Rat Anti-CD5 Recombinant Antibody (clone 53-7.3) (CAT#: NEUT-392CQ)
This antibody is a rat monoclonal antibody that binds specifically to mouse CD5, and it can neutralize the bioactivity of mouse CD5.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in WB
Western blot analysis of NEUT-392CQ-Fab was performed by loading recombinant mouse CD5 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with purified antibody and HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 2 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in ELISA
ELISA analysis of NEUT-392CQ-Fab was performed by coating with recombinant mouse CD5 protein. Then blocked with BSA and incubated with NEUT-392CQ-Fab. The HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Spleen cells or thymocyte membranes
- Host Species
- Rat
- Type
- Rat IgG2a, κ
- Specificity
- Mouse CD5
- Species Reactivity
- Mouse
- Clone
- 53-7.3
- Applications
- FC, IHC-Fr, IP, ELISA, Block
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD5"
Select a product category from the dropdown menu below to view related products.
Please select product type
Mouse Antibody Products
Immunotoxin Products
Chimeric Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-884 | Mouse Anti-CD5 Recombinant Antibody (clone Zolimomab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Mouse IgG1 |
| TAB-885 | Mouse Anti-CD5 Recombinant Antibody (clone Telimomab); Fab Fragment | FuncS, IF, Neut, ELISA, FC, IP, ICC | Mouse Fab |
| TAB-308LC | Anti-Human CD5 Recombinant Antibody (5D7) | Cyt, FC, FuncS | |
| TAB-308LC-S(P) | Anti-Human CD5 Recombinant Antibody scFv Fragment (5D7) | FC, SPR | |
| TAB-308LC-F(E) | Anti-Human CD5 Recombinant Antibody Fab Fragment (5D7) | FC, SPR |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for NEUT-392CQ. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NEUT-392CQ, RRID: AB_3111570)
Copy citation
Popular products with customers
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, SPR, Inhib, FuncS
-YJ-1.jpg)
Application: ELISA
-1.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, WB, Neut, Funcs, IHC, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Inhib, FuncS
Application: ELISA, FC, Neut, Inhib
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
