Anti-CSPG4 immunotoxin (scFv)-PE
CAT#: AGTO-G013E
This immunotoxin was achieved by conjugating/fusing the Anti-CSPG4 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating glioblastoma multiforme (GBM).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1933/1898_E12_17_cr5f68f3ec906de_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50008/782_C2_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16189/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, SK-MEL-30, U-138 MG, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173546-CSPG4
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Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- scFv-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- glioblastoma multiforme (GBM)
Target
- Alternative Names
- CSPG4; chondroitin sulfate proteoglycan 4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated); Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE39
- Gene ID
- 1464
- UniProt ID
- Q6UVK1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G013S | Anti-CSPG4 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study | |
| AGTO-G014E | Anti-CSPG4 immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-G014S | Anti-CSPG4 immunotoxin (IgG)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-102CT | Anti-Human CSPG4 Recombinant Antibody (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-S(P) | Anti-Human CSPG4 Recombinant Antibody scFv Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-F(E) | Anti-Human CSPG4 Recombinant Antibody Fab Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-103CT | Mouse Anti-CSPG4 Recombinant Antibody (TAB-103CT) | Funcs, FC, ELISA, WB | Mouse IgG |
| TAB-104CT | Mouse Anti-CSPG4 Recombinant Antibody (TAB-104CT) | IB, IF, IHC, FC | Mouse IgG |
| TAB-103CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-103CT-F(E)) | FC, ELISA, WB | Mouse Fab |
| TAB-104CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-104CT-F(E)) | FC | Mouse Fab |
| TAB-105CT-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-105CT-F(E)) | FC, ELISA, WB, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-550CQ | Mouse Anti-CSPG4 Recombinant Antibody (clone 9.2.27) | EM, Block, IP, FC, ICC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0840 | Hi-Affi™ Rabbit Anti-CSPG4 Recombinant Antibody (clone DS840AB) | WB, IHC, FC | Rabbit IgG |
| MOR-0105-FY | Rabbit Anti-CSPG4 Recombinant Antibody (clone AFY0076) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0070-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0070-LSX) | ELISA | Mouse IgG1 |
| HPAB-644-FY | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-644-FY) | FC, ELISA | Mouse IgG |
| HPAB-645-FY | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-645-FY) | FC, ELISA | Mouse IgG |
| HPAB-1039WJ | Human Anti-CSPG4 Recombinant Antibody (HPAB-1039WJ) | ELISA | Human IgG |
| VS-0723-WK105 | Human Anti-CSPG4 Recombinant Antibody (VS-0723-WK105) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0070-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0070-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0071-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0071-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0072-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0072-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0079-FY-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone M4-3 ML2); scFv Fragment | ELISA | Humanized scFv |
| HPAB-595-FY-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-595-FY-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0079-FY-F(E) | Human Anti-CSPG4 Recombinant Antibody (clone M4-3 ML2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1039WJ-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1039WJ-F(E)) | ELISA | Human Fab |
| HPAB-1040WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1040WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1041WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1041WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1042WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1042WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY61 | Human Anti-CSPG4 (clone D2A-1H10) scFv-Fc Chimera | ELISA, WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC269 | Recombinant Anti-CSPG4 Vesicular Antibody, EV Displayed (VS-0425-YC269) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1731 | Anti-Mouse CSPG4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1732 | Anti-Rat CSPG4 Immunohistochemistry Kit | IHC |
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