Anti-CSPG4 immunotoxin (scFv)-Sap
CAT#: AGTO-G013S
This immunotoxin was achieved by conjugating/fusing the Anti-CSPG4 scFv to saporin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells and enters cells by receptor-mediated endocytosis. Upon release of the conjugate from endosomes, the saporin toxin catalytically renders the large subunit ribosomes unable to bind the elongation factors and inhibits protein synthesis. It was designed for treatment of glioblastoma multiforme (GBM).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1933/1898_E12_17_cr5f68f3ec906de_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50008/782_C2_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16189/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, SK-MEL-30, U-138 MG, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173546-CSPG4
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Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- scFv-Sap
- Toxin Description
- Saporin is a so-called ribosome inactivating protein (RIP), due to its N-glycosidase activity, from the seeds of Saponaria officinalis (common name: soapwort).Saporin depurinate the ribosomal RNA in a specific position; rendering the large subunit of ribosomes unable to bind the elongation factors. The consequent arrest of protein synthesis is responsible for the cytotoxicity.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- glioblastoma multiforme (GBM)
Target
- Alternative Names
- CSPG4; chondroitin sulfate proteoglycan 4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated); saporin; saporin-6; saporin-S10; SAP
- Gene ID
- 1464
- UniProt ID
- Q6UVK1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G013E | Anti-CSPG4 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G014E | Anti-CSPG4 immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-G014S | Anti-CSPG4 immunotoxin (IgG)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-102CT | Anti-Human CSPG4 Recombinant Antibody (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-S(P) | Anti-Human CSPG4 Recombinant Antibody scFv Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-F(E) | Anti-Human CSPG4 Recombinant Antibody Fab Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-103CT | Mouse Anti-CSPG4 Recombinant Antibody (TAB-103CT) | Funcs, FC, ELISA, WB | Mouse IgG |
| TAB-104CT | Mouse Anti-CSPG4 Recombinant Antibody (TAB-104CT) | IB, IF, IHC, FC | Mouse IgG |
| TAB-103CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-103CT-F(E)) | FC, ELISA, WB | Mouse Fab |
| TAB-104CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-104CT-F(E)) | FC | Mouse Fab |
| TAB-105CT-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-105CT-F(E)) | FC, ELISA, WB, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-550CQ | Mouse Anti-CSPG4 Recombinant Antibody (clone 9.2.27) | EM, Block, IP, FC, ICC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0840 | Hi-Affi™ Rabbit Anti-CSPG4 Recombinant Antibody (clone DS840AB) | WB, IHC, FC | Rabbit IgG |
| MOR-0105-FY | Rabbit Anti-CSPG4 Recombinant Antibody (clone AFY0076) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0070-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0070-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0071-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0071-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0072-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0072-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0073-LSX-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC8); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0079-FY-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone M4-3 ML2); scFv Fragment | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0079-FY | Human Anti-CSPG4 Recombinant Antibody (clone M4-3 ML2) | ELISA | Humanized IgG1 |
| HPAB-644-FY | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-644-FY) | FC, ELISA | Mouse IgG |
| HPAB-645-FY | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-645-FY) | FC, ELISA | Mouse IgG |
| VS3-XY544 | Mouse Anti-CSPG4 Recombinant Antibody (clone 4E2) | ELISA, WB, FC | Mouse IgG1 |
| VS-0723-WK105 | Human Anti-CSPG4 Recombinant Antibody (VS-0723-WK105) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-645-FY-F(E) | Mouse Anti-CSPG4 Recombinant Antibody Fab Fragment (HPAB-645-FY-F(E)) | FC, ELISA | Mouse Fab |
| HPAB-1039WJ-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1039WJ-F(E)) | ELISA | Human Fab |
| HPAB-1040WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1040WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1041WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1041WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1042WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1042WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY61 | Human Anti-CSPG4 (clone D2A-1H10) scFv-Fc Chimera | ELISA, WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC269 | Recombinant Anti-CSPG4 Vesicular Antibody, EV Displayed (VS-0425-YC269) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1731 | Anti-Mouse CSPG4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1732 | Anti-Rat CSPG4 Immunohistochemistry Kit | IHC |
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