Human Anti-CSPG4 (clone D2A-1H10) scFv-Fc Chimera
CAT#: VS-0125-FY61
CSPG4 is highly expressed in tumor cells, including cancer stem cells, while its distribution in normal tissues is relatively limited. Therefore, it is considered an attractive target for cancer immunotherapy. CSPG4 is associated with the activation of several signaling pathways, including ERK and FAK, which play crucial roles in tumor cell proliferation, migration, and tumor progression. scFv-Fc can effectively inhibit tumor cell proliferation and migration in vitro, as well as in vivo, by blocking the ERK and FAK signaling pathways associated with CSPG4. scFv-Fc presents itself as a potential therapeutic agent that can target various tumors expressing CSPG4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1933/1898_E12_17_cr5f68f3ec906de_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50008/782_C2_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16189/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, SK-MEL-30, U-138 MG, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173546-CSPG4
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- D2A-1H10
- Applications
- ELISA, Western Blot, Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The scFv-FcD2A-1H10 demonstrates strong anti-tumor activity and safety, showing potential for application in the treatment of tumors expressing CSPG4.
Target
- Alternative Names
- Chondroitin Sulfate Proteoglycan 4; Melanoma-Associated Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan 4 (Melanoma-Associated); Melanoma Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan NG2; MCSP; MEL-CSPG
- Gene ID
- 1464
- UniProt ID
- Q6UVK1
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein; Extracellular side
Apical cell membrane; Single-pass type I membrane protein; Extracellular side
Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side
Cell surface
- Post Translation Modifications
- O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Glycosylation at Asn130, Asn348, Asn427, Asn685, Asn772, Ser995, Asn1131, Asn1202, Asn1364, Asn1449, Asn1645, Asn1909, Asn2016, Asn2034, Asn2040, and Asn2075
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect CSPG4_HUMAN (2028)
Glycosylation from GlyGen 19 sites, 25 N-linked glycans (9 sites), 4 O-linked glycans (3 sites)
- Protein Refseq
- XP_047288152.1; XP_054233345.1; NP_001888.2
- Function
- Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis.
May also inhibit neurite outgrowth and growth cone collapse during axon regeneration.
Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate.
Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity.
May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties.
May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.
Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins.
May promote retraction fiber formation and cell polarization through Rho GTPase activation.
May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1.
May activate FAK and ERK1/ERK2 signaling cascades.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Clone D2A-1H10"
- CAT
- Product Name
See other products for "CSPG4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1806z | Mouse Anti-CSPG4 Recombinant Antibody (clone 43C3) | WB, FC, IHC | Mouse IgG1, κ |
| VS3-WK1886 | Recombinant Rabbit Anti-Mouse CSPG4 Capture Antibody (clone R53-2Z-1) | ELISA | Rabbit IgG |
| VS3-WK1887 | Recombinant Rabbit Anti-Mouse CSPG4 Detection Antibody (clone R14-7S-4) | ELISA | Rabbit IgG |
| VS3-WK1888 | Recombinant Rabbit Anti-CSPG4 Capture Antibody (clone R52-6C-8) | ELISA | Rabbit IgG |
| VS3-WK1889 | Recombinant Rabbit Anti-CSPG4 Detection Antibody (clone R35-5A-8) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G013E | Anti-CSPG4 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G013S | Anti-CSPG4 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study | |
| AGTO-G014E | Anti-CSPG4 immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
| AGTO-G014S | Anti-CSPG4 immunotoxin (IgG)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-102CT | Anti-Human CSPG4 Recombinant Antibody (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-S(P) | Anti-Human CSPG4 Recombinant Antibody scFv Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| TAB-102CT-F(E) | Anti-Human CSPG4 Recombinant Antibody Fab Fragment (D2A-1H10) | ELISA, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-103CT-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (TAB-103CT-S(P)) | FC, ELISA, WB | Mouse scFv |
| TAB-104CT-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (TAB-104CT-S(P)) | FC | Mouse scFv |
| TAB-103CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-103CT-F(E)) | FC, ELISA, WB | Mouse Fab |
| TAB-104CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-104CT-F(E)) | FC | Mouse Fab |
| TAB-105CT-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-105CT-F(E)) | FC, ELISA, WB, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-550CQ | Mouse Anti-CSPG4 Recombinant Antibody (clone 9.2.27) | EM, Block, IP, FC, ICC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0840 | Hi-Affi™ Rabbit Anti-CSPG4 Recombinant Antibody (clone DS840AB) | WB, IHC, FC | Rabbit IgG |
| MOR-0105-FY | Rabbit Anti-CSPG4 Recombinant Antibody (clone AFY0076) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0070-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0070-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0071-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0071-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0072-LSX-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-0072-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0073-LSX-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC8); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0074-LSX-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC12); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0079-FY-F(E) | Human Anti-CSPG4 Recombinant Antibody (clone M4-3 ML2); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1039WJ-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1039WJ-F(E)) | ELISA | Human Fab |
| HPAB-1040WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1040WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1041WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1041WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1042WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1042WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC269 | Recombinant Anti-CSPG4 Vesicular Antibody, EV Displayed (VS-0425-YC269) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1731 | Anti-Mouse CSPG4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1732 | Anti-Rat CSPG4 Immunohistochemistry Kit | IHC |
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