Human Anti-CSPG4 (clone D2A-1H10) scFv-Fc Chimera (CAT#: VS-0125-FY61)

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Species Reactivity
• Human

• Clone
• D2A-1H10

• Applications
• ELISA, Western Blot, Inhibition

• Related Disease
• Cancer

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• No preservatives

• Stabilizer
• No Stabilizers

• Sterility
• 0.2 μM filtered

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• The scFv-FcD2A-1H10 demonstrates strong anti-tumor activity and safety, showing potential for application in the treatment of tumors expressing CSPG4.

• Alternative Names
• Chondroitin Sulfate Proteoglycan 4; Melanoma-Associated Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan 4 (Melanoma-Associated); Melanoma Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan NG2; MCSP; MEL-CSPG

• Gene ID
• 1464

• UniProt ID
• Q6UVK1

• Cellular Localization
• Cell membrane; Single-pass type I membrane protein; Extracellular side
  Apical cell membrane; Single-pass type I membrane protein; Extracellular side
  Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side
  Cell surface

• Post Translation Modifications
• O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation.
  Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
  Glycosylation at Asn130, Asn348, Asn427, Asn685, Asn772, Ser995, Asn1131, Asn1202, Asn1364, Asn1449, Asn1645, Asn1909, Asn2016, Asn2034, Asn2040, and Asn2075
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyConnect CSPG4_HUMAN (2028)
  Glycosylation from GlyGen 19 sites, 25 N-linked glycans (9 sites), 4 O-linked glycans (3 sites)

• Protein Refseq
• XP_047288152.1; XP_054233345.1; NP_001888.2

• Function
• Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis.
  May also inhibit neurite outgrowth and growth cone collapse during axon regeneration.
  Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate.
  Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity.
  May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties.
  May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.
  Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins.
  May promote retraction fiber formation and cell polarization through Rho GTPase activation.
  May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1.
  May activate FAK and ERK1/ERK2 signaling cascades.

Secondary Antibody

• 2ND-029CQ
• Goat Anti-Human IgG Fc Antibody

Isotype Control

• MOB-200CQ
• Human IgG Fc Isotype Control Antibody

• TAB-102CT
• Anti-Human CSPG4 Recombinant Antibody (D2A-1H10)

• TAB-102CT-S(P)
• Anti-Human CSPG4 Recombinant Antibody scFv Fragment (D2A-1H10)

• TAB-102CT-F(E)
• Anti-Human CSPG4 Recombinant Antibody Fab Fragment (D2A-1H10)

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