Anti-CSPG4 immunotoxin (IgG)-PE (CAT#: AGTO-G014E)
This immunotoxin was achieved by conjugating/fusing the Anti-CSPG4 monoclonal antibody to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating rhabdomyosarcoma.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1933/1898_E12_17_cr5f68f3ec906de_selected.jpg
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1933/1898_E12_17_cr5f68f3ec906de_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50008/782_C2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50008/782_C2_1_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/ihc_selected.jpg
Figure 4 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16189/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16189/ihc_selected.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2951/10455_A_8_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (BJ, SK-MEL-30, U-138 MG, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173546-CSPG4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173546-CSPG4
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- IgG-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- rhabdomyosarcoma
Target
- Alternative Names
- CSPG4; chondroitin sulfate proteoglycan 4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated); Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE40
- Gene ID
- 1464
- UniProt ID
- Q6UVK1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G013E | Anti-CSPG4 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G013S | Anti-CSPG4 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study | |
| AGTO-G014S | Anti-CSPG4 immunotoxin (IgG)-Sap | Cytotoxicity assay, Function study |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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