Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-014)
CAT#: PNBL-014
Anti-E. coli DHFR VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
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Specifications
- Immunogen
- E. coli Dihydrofolate reductase
- Host Species
- Llama
- Derivation
- Llama
- Type
- Llama VHH
- Species Reactivity
- E. coli
- Clone
- PNBL-014
- Applications
- This product is suitable for use in WB and most other immunological methods.
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- DHFR; Dihydrofolate reductase
- Gene ID
- 13906554
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Effective in Western Blotting
We successfully used this antibody in Western Blotting. The results were clear and reproducible, helping us identify DHFR with precision.
Effective for Immunoprecipitation
The antibody was effective in immunoprecipitation assays, helping us pull down E. coli DHFR with high efficiency.
Minimal Cross-Reactivity
We tested for cross-reactivity and found none. This antibody is highly specific to E. coli DHFR, which is crucial for our work.
Q&As
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Is it easy to purify this antibody?
A: Standard protein purification methods such as affinity chromatography can effectively purify the antibody. Since it's a single domain antibody, the product is frequently quite pure.
-
Can this antibody be conjugated to fluorescent dyes?
A: Indeed, typical conjugation techniques seen in the literature can be used to conjugate the recombinant anti-E. coli DHFR VHH single domain antibody to a variety of fluorescent colors.
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What are the main research applications for this antibody?
A: Immunoassays such as immunoprecipitation and Western blotting are widely used, especially in E. coli research and related investigations.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DHFR"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812z | Mouse Anti-DHFR Recombinant Antibody (clone 30C7) | WB, ICC, IHC | Mouse IgG2b |
| MOB-0052CT | Recombinant Mouse anti-Human DHFR Monoclonal antibody (4B22) | IHC-P, WB | |
| ZG-0636J | Rabbit Anti-DHFR Recombinant Antibody (clone 9B2) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-XY3331 | Rabbit Anti-DHFR Recombinant Antibody (clone R05-2C7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-013 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-013) | FACS, ELISA, FuncS | Llama VHH |
| PNBL-074 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-074) | NMR, ELISA | Llama VHH |
| PNBL-075 | Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075) | WB, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0976 | Hi-Affi™ Rabbit Anti-DHFR Recombinant Antibody (clone DS976AB) | ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY163 | Mouse Anti-NHP DHFR Recombinant Antibody (clone OTI6G7) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1984 | Anti-DHFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY1985 | Anti-Mouse DHFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY1986 | Anti-Canine DHFR Immunohistochemistry Kit | IHC |
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