Rabbit Anti-DHFR Recombinant Antibody (clone R05-2C7) (CAT#: VS3-XY3331)
The rabbit recombinant antibody specifically recognizes DHFR (Dihydrofolate reductase), which catalyzes an essential reaction for de novo glycine and purine synthesis. It reacts with human, mouse and rat DHFR. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DHFR
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-2C7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Inherited Cancer-Predisposing Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- DYR; DHFR1; DHFRP1
- Gene ID
- 1719
- UniProt ID
- P00374
- Long Name
- Dihydrofolate reductase
- Cellular Localization
- Mitochondrion, Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys19, Lys81, Lys133, Lys158, Lys174, Lys179, and Lys185 (NX_P00374 [NX_P00374-1])
Modification sites at PhosphoSitePlus
- Protein Refseq
- NP_000782.1; NP_001277283.1
- Function
- Key enzyme in folate metabolism.
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DHFR"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812z | Mouse Anti-DHFR Recombinant Antibody (clone 30C7) | WB, ICC, IHC | Mouse IgG2b |
| MOB-0052CT | Recombinant Mouse anti-Human DHFR Monoclonal antibody (4B22) | IHC-P, WB | |
| ZG-0636J | Rabbit Anti-DHFR Recombinant Antibody (clone 9B2) | ELISA, WB, IHC, IP | Rabbit IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-013 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-013) | FACS, ELISA, FuncS | Llama VHH |
| PNBL-014 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-014) | WB, FuncS | Llama VHH |
| PNBL-074 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-074) | NMR, ELISA | Llama VHH |
| PNBL-075 | Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075) | WB, FuncS | Llama VHH |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0976 | Hi-Affi™ Rabbit Anti-DHFR Recombinant Antibody (clone DS976AB) | ICC, IF, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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