Anti-Mouse DHFR Immunohistochemistry Kit
CAT#: VS-0525-XY1985
Our DHFR IHC kit provides all-inclusive reagents for detecting this key enzyme in folate metabolism and DNA synthesis. Suitable for paraffin and frozen sections across species, it supports cancer, metabolism, and cell proliferation research with clear and reproducible staining results.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/766_F2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51465/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37129/73545_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000228716-DHFR
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- DHFR
- Primary Antibody
- Rabbit Anti-DHFR Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "DHFR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812z | Mouse Anti-DHFR Recombinant Antibody (clone 30C7) | WB, ICC, IHC | Mouse IgG2b |
| MOB-0052CT | Recombinant Mouse anti-Human DHFR Monoclonal antibody (4B22) | IHC-P, WB | |
| ZG-0636J | Rabbit Anti-DHFR Recombinant Antibody (clone 9B2) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-XY3331 | Rabbit Anti-DHFR Recombinant Antibody (clone R05-2C7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-013 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-013) | FACS, ELISA, FuncS | Llama VHH |
| PNBL-014 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-014) | WB, FuncS | Llama VHH |
| PNBL-074 | Recombinant Anti-E. coli DHFR VHH Single Domain Antibody (PNBL-074) | NMR, ELISA | Llama VHH |
| PNBL-075 | Recombinant Anti-E. coli dhfr VHH Single Domain Antibody (PNBL-075) | WB, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0976 | Hi-Affi™ Rabbit Anti-DHFR Recombinant Antibody (clone DS976AB) | ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY163 | Mouse Anti-NHP DHFR Recombinant Antibody (clone OTI6G7) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1984 | Anti-DHFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY1986 | Anti-Canine DHFR Immunohistochemistry Kit | IHC |
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