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    Hi-Affi™ Rabbit Anti-E2F1 Recombinant Antibody (clone DS1080AB) (CAT#: MOR-1080)

    Recombinant Rabbit anti-E2F1 monoclonal antibody for WB, ICC/IF, IP, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human rectum
    Figure 3 IHC staining of human rectum
    (Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
    Figure 4 IHC staining of human colon
    Figure 4 IHC staining of human colon
    (Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
    Figure 10 Bone marrow
    Figure 10 Bone marrow
    (Hematopoietic cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1

    Specifications

    • Immunogen
    • Synthetic peptide within Human E2F1 aa 350 to the C-terminus (Cysteine residue).
    • Host Species
    • Rabbit
    • Derivation
    • Phage display library
    • Type
    • Rabbit IgG
    • Specificity
    • Human E2F1
    • Species Reactivity
    • Mouse, Rat, Human
    • Clone
    • DS1080AB
    • Applications
    • WB, ICC, IF, IP, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • >95% as determined by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • E2F Transcription Factor 1; Retinoblastoma-Associated Protein 1; Retinoblastoma-Binding Protein 3; PRB-Binding Protein E2F-1; RBAP-1; RBBP-3; RBBP3; E2F-1; PBR3; RBAP1; RBP3

    Related Resources

    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "E2F1"

    Select a product category from the dropdown menu below to view related products.
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    CAT Product Name Application Type
    MOB-4001z Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) WB, IP, IF, ELISA Mouse IgG2b, κ
    MOB-0156CT Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) ICC/IF, IP, WB
    ZG-0641J Rabbit Anti-E2F1 Recombinant Antibody (clone 1D12) ELISA, WB, IHC, IF, FC Rabbit IgG
    VS3-QX336 Mouse Anti-E2F1 Recombinant Antibody (clone 4G8-2C2-C12) WB, IF, IP Mouse IgG2b
    VS3-FY466 Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) WB, IHC-F, IHC-P, ICC, IF, IP Rabbit IgG

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