Anti-Human EFNB2 Recombinant Antibody (2B1) (CAT#: TAB-389MZ)

This novel antibody 2B1 against ephrin B2 may be used to detect the protein and used as a medicament for inhibiting angiogenesis and lymphangiogenesis in the treatment studies of diseases in which these processes are implicated, for example, cancer.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Determination of the specificity of the scFv anti-ephrin B2 selected against different ephrin B by immunoblot.

Figure 1 Determination of the specificity of the scFv anti-ephrin B2 selected against different ephrin B by immunoblot.

Block

Figure 2 Study Blocking the Binding of Ephrin B2 to Eph B4.

Figure 2 Study Blocking the Binding of Ephrin B2 to Eph B4.

Graph showing the percent of binding of ephrin B2 to Eph B4. Binding inhibition tests of ephrin B2 to Eph B4 in the presence of scFv anti-ephrin B2 by surface plasmon resonance. Serial dilutions of scFv B11 (•) or 2B1 (∘) were mixed with ephrin B2 and injected over Eph B4 immobilized on a chip. The relative amount of ephrin B2 bound to Eph B4 was measured immediately after injection of each sample and plotted as a function of the concentration of scFv.

Inhib

Figure 3 Inhibitory Effect of B11 and 2B1 on Tubular Structures Formation in Vitro by Endothelial Cells HUVEC in Matrigel.

Figure 3 Inhibitory Effect of B11 and 2B1 on Tubular Structures Formation in Vitro by Endothelial Cells HUVEC in Matrigel.

Photomicrographs (magnification 4×) representative of tubules formation at 6 hours of cultivate HUVEC cells on Matrigel in the presence of B11, 2B1 or an irrelevant scFv as negative control (C−). As a positive control we used VEGF. The graph shows the quantification of tubules formation. Each treatment was repeated at least three times and corresponding values (mean+/−standard deviation) are plotted as percentage of the tubules formed in the presence of the antibodies tested related to the control without any treatment. *=p<0.0001.

FuncS

Figure 4 Analysis of the of Cell Lateral Migration Inhibition by In Vitro Wound Healing Assay.

Figure 4 Analysis of the of Cell Lateral Migration Inhibition by In Vitro Wound Healing Assay.

HUVEC cells were grown to confluence and a wound was performed in the cell monolayer. Then, cells were incubated in serum free medium in the absence (control) or in presence of VEGF as migration stimulus. The lateral migration of the cells was monitored for 24 hours in the presence of the scFv anti-ephrin B2, B11 or 2B1, or an irrelevant scFv as negative control.

FuncS

Figure 5 Inhibition of Tumour Growth in Mice Xenograft with Pancreatic Carcinoma Cells (BxPC3) and Treated with B11 or 2B1.

Figure 5 Inhibition of Tumour Growth in Mice Xenograft with Pancreatic Carcinoma Cells (BxPC3) and Treated with B11 or 2B1.

The graph shows the average tumour volumes and standard deviation at each point for each group (n=8) treated with 20 mg/kg of B11 (┐), 2B1 (▪) or untreated (C−) (♦).


Specifications

  • Host Species
  • Human
  • Derivation
  • Phage M13 library
  • Type
  • Human antibody
  • Specificity
  • Human
  • Clone
  • 2B1
  • Applications
  • Block, FuncS, Inhib, WB
  • Related Disease
  • Cancers such as pancreatic, colon or lung cancer

Applications

  • Application Notes
  • This antibody has been reported for use in Blocking, Functional Assay, Inhibition, Western Blot.

Target

  • Alternative Names
  • EFNB2; ephrin-B2; HTKL; EPLG5; Htk-L; LERK5; LERK-5; HTK ligand; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 2B1"

See other products for "EFNB2"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1100 Hi-Affi™ Rabbit Anti-EFNB2 Recombinant Antibody (clone DS1100AB) WB, ICC, IF Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for TAB-389MZ. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare