Anti-Human EFNB2 Recombinant Antibody (B11)
CAT#: TAB-390MZ
This novel antibody against ephrin B2 may be used to detect the protein and used as a medicament for inhibiting angiogenesis and lymphangiogenesis in the treatment of diseases in which these processes are implicated, for example, cancer.
Published Data
Gene Expression
WB
Figure 1 Determination of the specificity of the scFv anti-ephrin B2 selected against different ephrin B by immunoblot.
Block
Figure 2 Study Blocking the Binding of Ephrin B2 to Eph B4.
Graph showing the percent of binding of ephrin B2 to Eph B4. Binding inhibition tests of ephrin B2 to Eph B4 in the presence of scFv anti-ephrin B2 by surface plasmon resonance. Serial dilutions of scFv B11 (•) or 2B1 (∘) were mixed with ephrin B2 and injected over Eph B4 immobilized on a chip. The relative amount of ephrin B2 bound to Eph B4 was measured immediately after injection of each sample and plotted as a function of the concentration of scFv.
Inhib
Figure 3 Inhibitory Effect of B11 and 2B1 on Tubular Structures Formation in Vitro by Endothelial Cells HUVEC in Matrigel.
Photomicrographs (magnification 4×) representative of tubules formation at 6 hours of cultivate HUVEC cells on Matrigel in the presence of B11, 2B1 or an irrelevant scFv as negative control (C−). As a positive control we used VEGF. The graph shows the quantification of tubules formation. Each treatment was repeated at least three times and corresponding values (mean+/−standard deviation) are plotted as percentage of the tubules formed in the presence of the antibodies tested related to the control without any treatment. *=p<0.0001.
FuncS
Figure 4 Analysis of the of Cell Lateral Migration Inhibition by In Vitro Wound Healing Assay.
HUVEC cells were grown to confluence and a wound was performed in the cell monolayer. Then, cells were incubated in serum free medium in the absence (control) or in presence of VEGF as migration stimulus. The lateral migration of the cells was monitored for 24 hours in the presence of the scFv anti-ephrin B2, B11 or 2B1, or an irrelevant scFv as negative control.
FuncS
Figure 5 Inhibition of Tumour Growth in Mice Xenograft with Pancreatic Carcinoma Cells (BxPC3) and Treated with B11 or 2B1.
The graph shows the average tumour volumes and standard deviation at each point for each group (n=8) treated with 20 mg/kg of B11 (┐), 2B1 (▪) or untreated (C−) (♦).
FuncS
Figure 6 B11 is Able to Inhibit Tumour Growth in Mice Xenografted with colon carcinoma cells (SW620).
The graphs show the mean tumour volumes (upper panels) and the mean fluorescence intensities (lower panels) with standard deviation at each point in the groups treated with 20 mg/kg of B11 and control groups treated.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/47_A4_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9368/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_5_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_5_6.jpg
Normal Tissue
Figure 9 Skin
Endothelial cells
Staining: Medium
Intensity: Moderate
Fibrohistiocytic cells
Staining: Medium
Intensity: Moderate
Lymphocytes
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_1.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, OE19, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125266-EFNB2
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Specifications
- Host Species
- Human
- Derivation
- Phage M13 library
- Type
- Human antibody
- Specificity
- Human
- Clone
- B11
- Applications
- Block, FuncS, Inhib, WB
- Related Disease
- Cancers such as pancreatic, colon or lung cancer
Applications
- Application Notes
- This antibody has been reported for use in Blocking, Functional Assay, Inhibition, Western Blot.
Target
REVIEWS AND Q&AS
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Effective with secondary antibodies.
This antibody exhibits great compatibility with a wide variety of secondary antibodies, resulting in robust and targeted signals in conjugated experiments.
Operating Effectively at Low Concentrations
Even at modest concentrations, this antibody produces effective and satisfactory outcomes. It works best in small amounts and is incredibly efficient.
Easy Protocol Enhancement
Using this antibody, procedure optimization was simple. It required little troubleshooting to obtain the optimum outcomes thanks to the accurate information.
Q&As
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What safety measures need to be followed when working with antibodies?
A: Wear appropriate PPE, such as gloves and lab coats, handle biohazardous products in a biosafety cabinet when needed, and safely dispose of them.
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How can I lessen the proteolytic degradation of my samples?
A: To avoid proteolytic degradation, add protease inhibitors to your lysis buffers and maintain samples on ice during processing.
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Why is the validation of antibodies important?
A: By confirming that the antibody binds precisely to its target epitope, validation produces results that are reliable and consistent throughout a variety of trials and environments.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304 | Recombinant Anti-human EFNB2 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-304 | Recombinant Human Anti-human EFNB2 Antibody | WB, IHC, FuncS | IgG |
| HPAB-0164-LSX | Human Anti-EFNB2 Recombinant Antibody (clone A11) | ELISA | Human IgG |
| HPAB-0165-LSX | Human Anti-EFNB2 Recombinant Antibody (clone B1) | ELISA | Human IgG |
| MRO-0539-CN | Rabbit Anti-EFNB2 Recombinant Antibody (clone JM53-21) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304-F(E) | Recombinant Anti-human EFNB2 Antibody Fab Fragment | IP, FuncS | Fab |
| MHH-304-F(E) | Recombinant Human Anti-human EFNB2 Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| HPAB-0164-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone A11); Fab Fragment | ELISA | Human Fab |
| HPAB-0165-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone B1); Fab Fragment | ELISA | Human Fab |
| HPAB-0166-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone D1); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-304-S(P) | Recombinant Anti-human EFNB2 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-304-S(P) | Recombinant Human Anti-human EFNB2 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-0164-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone A11); scFv Fragment | ELISA | Human scFv |
| HPAB-0165-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone B1); scFv Fragment | ELISA | Human scFv |
| HPAB-0166-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone D1); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-389MZ | Anti-Human EFNB2 Recombinant Antibody (2B1) | Block, FuncS, Inhib, WB | Human antibody |
| TAB-391MZ | Anti-Human EFNB2 Recombinant Antibody (A1) | ELISA | Human antibody |
| TAB-392MZ | Anti-Human EFNB2 Recombinant Antibody (E4 ) | ELISA | Human antibody |
| TAB-393MZ | Anti-Human EFNB2 Recombinant Antibody (TAB-393MZ) | IHC, ELISA | Human antibody |
| TAB-394MZ | Anti-Human EFNB2 Recombinant Antibody (TAB-394MZ) | IHC, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1100 | Hi-Affi™ Rabbit Anti-EFNB2 Recombinant Antibody (clone DS1100AB) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC163 | Recombinant Anti-EFNB2 Vesicular Antibody, EV Displayed (VS-0425-YC163) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2176 | Anti-EFNB2 Immunohistochemistry Kit | IHC |
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