Recombinant Anti-human EFNB2 Antibody scFv Fragment

CAT#: MOB-304-S(P)

Recombinant Mouse Antibody scFv Fragment prepared by a genetic engineering technique which specifically reacts with Human EFNB2, expressed in E. coli.

Gene Expression
Figure 1 IF staining of human cell line U-2 OS Figure 2 IF staining of human cell line U-251 MG Figure 3 IHC staining of human kidney Figure 4 Cerebral cortex Figure 5 Colon Figure 6 Liver Figure 7 Kidney Figure 8 Testis Figure 9 Skin Figure 10 RNA cell line category: Cell line enhanced (AF22, OE19, SH-SY5Y)

Specifications

  • Immunogen
  • Human ephrin-B2
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • scFv
  • Specificity
  • Tested positive against native Human ephrin-B2
  • Species Reactivity
  • Human
  • Applications
  • Formats of immunological tests: Immunofluorescence; Neutralization; Functional Study

Product Property

  • Purity
  • >95% as determined by analysis by RP-HPLC
  • Storage
  • Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.

Target

  • Alternative Names
  • EFNB2; ephrin-B2; HTKL; EPLG5; Htk-L; LERK5; LERK-5; HTK ligand; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5
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Breast cancer biomarkers at key points during disease progression
Unparalleled Sensitivity in Detection
We were impressed by the antibody's ability to detect low levels of EFNB2 in our samples. It consistently outperformed other antibodies, making it our go-to choice for sensitive assays. This has been particularly beneficial in early disease detection studies where protein levels are minimal.
Breast cancer biomarkers at key points during disease progression
High Specificity Ensures Reliable Results
The specificity of this antibody is outstanding. It binds exclusively to EFNB2 without cross-reactivity, which has greatly improved the accuracy of our experiments. This reliability has increased our confidence in the data and accelerated our research progress.
Breast cancer biomarkers at key points during disease progression
Enhancing Drug Discovery Efforts
In our search for EFNB2 inhibitors, this antibody has been a critical component of our screening assays. Its sensitivity and specificity have allowed us to accurately assess the impact of various compounds on EFNB2 activity. This has streamlined our drug discovery process and improved our success rate.

Q&As

  1. How does the anti-human EFNB2 scFv antibody enable precise localization in single-cell immunofluorescence analysis?

    A: In single-cell immunofluorescence analysis, the anti-human EFNB2 scFv antibody binds specifically to the EFNB2 protein within individual cells. Its small scFv fragment allows for better tissue penetration and minimal steric hindrance, resulting in highly precise localization of EFNB2. This precision enables quantitative analysis of EFNB2 expression levels and distribution patterns at the single-cell level, providing detailed insights into cellular functions and signaling mechanisms.

  2. How does EFNB2 contribute to neurodegenerative diseases, and how can this antibody aid in research?

    A: EFNB2 is involved in neurodevelopment and synaptic plasticity. Abnormal expression or post-translational modifications of EFNB2 have been associated with neurodegenerative diseases like Alzheimer's and Parkinson's. The anti-human EFNB2 scFv antibody enables researchers to detect and quantify these alterations in EFNB2, helping to elucidate its role in disease onset and progression. This aids in understanding the molecular mechanisms underlying neurodegeneration and could inform therapeutic strategies.

  3. What sensitivity advantages does this antibody have in detecting EFNB2 compared to similar products?

    A: The anti-human EFNB2 scFv antibody exhibits high sensitivity due to its strong affinity for EFNB2 and low non-specific binding. It can detect even low levels of EFNB2, making it superior for applications requiring precise quantification. This sensitivity is particularly beneficial in early disease detection, when EFNB2 expression changes may be subtle but significant.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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