Hi-Affi™ Rabbit Anti-EFNB2 Recombinant Antibody (clone DS1100AB)
CAT#: MOR-1100
Recombinant Rabbit anti-EFNB2 monoclonal antibody for WB, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/47_A4_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9368/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_5_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_A_5_6.jpg
Normal Tissue
Figure 9 Skin
Endothelial cells
Staining: Medium
Intensity: Moderate
Fibrohistiocytic cells
Staining: Medium
Intensity: Moderate
Lymphocytes
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8999/26099_B_7_1.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, OE19, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125266-EFNB2
❮
❯
Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human EFNB2
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS1100AB
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOB-304-F(E) | Recombinant Anti-human EFNB2 Antibody Fab Fragment | IP, FuncS | Fab |
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| HPAB-0164-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone A11); Fab Fragment | ELISA | Human Fab |
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| HPAB-0166-LSX-F(E) | Human Anti-EFNB2 Recombinant Antibody (clone D1); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
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| MOB-304-S(P) | Recombinant Anti-human EFNB2 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| MHH-304-S(P) | Recombinant Human Anti-human EFNB2 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-0164-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone A11); scFv Fragment | ELISA | Human scFv |
| HPAB-0165-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone B1); scFv Fragment | ELISA | Human scFv |
| HPAB-0166-LSX-S(P) | Human Anti-EFNB2 Recombinant Antibody (clone D1); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-389MZ-F(E) | Anti-Human EFNB2 Recombinant Antibody Fab Fragment (2B1) | ELISA | Human antibody |
| TAB-390MZ-F(E) | Anti-Human EFNB2 Recombinant Antibody Fab Fragment (B11) | ELISA | Human antibody |
| TAB-391MZ-F(E) | Anti-Human EFNB2 Recombinant Antibody Fab Fragment (A1) | ELISA | Human antibody |
| TAB-392MZ-F(E) | Anti-Human EFNB2 Recombinant Antibody Fab Fragment (E4 ) | ELISA | Human antibody |
| TAB-393MZ-F(E) | Anti-Human EFNB2 Recombinant Antibody Fab Fragment (TAB-393MZ-F(E)) | IHC, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC163 | Recombinant Anti-EFNB2 Vesicular Antibody, EV Displayed (VS-0425-YC163) | Block, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2176 | Anti-EFNB2 Immunohistochemistry Kit | IHC |
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