Anti-Human FLT3 Recombinant Antibody (IMC-EB10) (CAT#: TAB-291CL)
Recombinant Human monoclonal antibody expressed in CHO binding to Human FLT3. IMC-EB10 is an anti-FLT3 monoclonal antibody, prolongs survival and reduces nonobese diabetic/severe combined immunodeficient engraftment of some acute lymphoblastic leukemia cell lines and primary leukemic samples.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human FLT3 Therapeutic Antibody (TAB-291CL) in DB
Dot Blot analysis of TAB-291CL was performed by coating with human FLT3 protein (His tag).
TAB-291CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 6000)
Figure 2 Anti-Human FLT3 Therapeutic Antibody (TAB-291CL) in WB
Western blot analysis of TAB-291CL was performed with human FLT3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-291CL and HRP goat anti-Human IgG was used as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.2 μg)
Lane 2: Reduced antigen (0.4 μg)
Lane 3: Reduced antigen (0.8 μg)
Figure 3 Anti-Human FLT3 Therapeutic Antibody (TAB-291CL) in ELISA
ELISA analysis of TAB-291CL was performed by coating with human FLT3 protein (His tag). Then blocked with BSA and incubated with TAB-291CL. The HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Antibody
- Species Reactivity
- Human
- Applications
- WB, IHC, FC, Cyt, ELISA
- Related Disease
- Acute myeloid leukemia
Product Property
- Purity
- >95% by HPLC
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunohistochemistry, Flow Cytometry, Cytotoxicity and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- FLT3; fms-related tyrosine kinase 3; FLK2; STK1; CD135; FLK-2; receptor-type tyrosine-protein kinase FLT3; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine
- Gene ID
- 2322
- UniProt ID
- P36888
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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MOB-1630z | Mouse Anti-FLT3 Recombinant Antibody (clone 23F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
HPAB-0103-YC | Human Anti-FLT3 Recombinant Antibody (HPAB-0103-YC) | ELISA, Block, FC | Humanized IgG1, κ |
HPAB-0182CQ | Human Anti-FLT3 Recombinant Antibody (clone D4-3) | ELISA, Neut | Human IgG1, κ |
HPAB-0183CQ | Human Anti-FLT3 Recombinant Antibody (clone NC7) | ELISA, Neut | Human IgG1, κ |
VS3-XY714 | Mouse Anti-FLT3 Recombinant Antibody (clone 7B7C3) | ELISA, WB, IHC | Mouse IgG1 |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-291CL, RRID: AB_3111902)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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