Recombinant Anti-human GLI3 Intrabody [(D-Arg)9]
CAT#: IAB-B069(A)
Recombinant anti-GLI3 intrabody linked to (D-Arg)9 works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/61_A4_2_selected.jpg
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Specifications
- Immunogen
- human GLI family zinc finger 3
- Host Species
- llama
- Type
- vhh-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GLI3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396 | Recombinant Anti-human GLI3 Antibody | WB, IP, FuncS | IgG |
| MHH-396 | Recombinant Human Anti-human GLI3 Antibody | ELISA, WB, Neut, FuncS | IgG |
| MOB-0773CT | Recombinant Mouse anti-Human GLI3 Monoclonal antibody (EML508) | WB | |
| MRO-0659-CN | Rabbit Anti-GLI3 Recombinant Antibody (clone JG83-39) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-F(E) | Recombinant Anti-human GLI3 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| MHH-396-F(E) | Recombinant Human Anti-human GLI3 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-S(P) | Recombinant Anti-human GLI3 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-396-S(P) | Recombinant Human Anti-human GLI3 Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B069(G) | Recombinant Anti-human GLI3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| IAB-B069(T) | Recombinant Anti-human GLI3 Intrabody [Tat] | ELISA, WB, ICC, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1452 | Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY922 | Anti-GLI3 Immunohistochemistry Kit | IHC |
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