How does the antibody ensure specificity toward GPNMB in various assays?

We designed the Anti-GPNMB Recombinant Antibody (clone Glembatumumab) with a human IgG2, κ framework to specifically bind to human GPNMB. Its performance has been validated in Western Blot, IF, IP, and ELISA assays. Detailed purity data and titration recommendations help achieve consistent results across different experiments.

What purification and validation methods are applied to this antibody?

We use stringent purification techniques, ensuring that the antibody exceeds 95% purity, as verified by SDS-PAGE and HPLC analyses. The antibody has been tested in multiple applications including Neutralization and Functional Assays, and the comprehensive product data provided assists researchers in effectively employing it for GPNMB detection.

Human Anti-GPNMB Recombinant Antibody (clone Glembatumumab)

CAT#: TAB-751

This product is a human IgG2, κ antibody that can recognize human GPNMB.

Tested Data Gene Expression

SDS-PAGE

Figure 1 GPNMB Recombinant Antibody (TAB-751) in SDS-PAGE

SDS-PAGE analysis of TAB-751 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions.

SEC-HPLC

Figure 2 GPNMB Recombinant Antibody (TAB-751) in HPLC

The purity of TAB-751 was greater than 95% as determined by SEC-HPLC.

Figure 3 GPNMB Recombinant Antibody (TAB-751) in WB

Western blot analysis was performed by loading 0.1 µg (lane 1), 0.1 µg(lane 2), and 0.6 µg (lane 3) recombinant human GPNMB.

The secondary antibody: HRP-Anti-Human IgG (H+L)

Dot Blot

Figure 4 GPNMB Recombinant Antibody (TAB-751) Dot Blot

Dot Blot analysis of TAB-751 was performed by coating with human GPNMB antigen.
TAB-751 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

ELISA

Figure 5 GPNMB Recombinant Antibody (TAB-751) in ELISA

ELISA analysis of TAB-751 was performed by coating with recombinant human GPNMB antigen.

The secondary antibody: HRP-Anti-Human IgG (H+L)

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RNA Expression Figure 1 RNA cell line category: Cell line enriched (ASC diff)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136235-GPNMB

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
Human IgG2, κ

Specificity
Human GPNMB

Species Reactivity
Human

Clone
Glembatumumab

Applications
WB, IF, IP, Neut, FuncS, ELISA, FC

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Western Blot, Immunofluorescence, Immunoprecipitation, Neutralization, Functional Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

Alternative Names
glycoprotein nmb; NMB; HGFIN; PLCA3; transmembrane glycoprotein NMB; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1 type; osteoactivin; transmembrane glycoprotein HGFIN

Gene ID
10457

UniProt ID
Q14956

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Customer Review

There are currently no Customer reviews or questions for TAB-751. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Excellent Specificity

I recently tested the Anti-GPNMB Recombinant Antibody (clone Glembatumumab) in a series of immunofluorescence and Western Blot assays. Its high purity and specific binding to human GPNMB were clearly demonstrated. The detailed COA and recommended dilutions contributed to accurate detection and reproducible data in my research.

Detailed Product Info

The antibody targeting GPNMB provided impressive results in multiple assays including ELISA and neutralization tests. I found the product information to be comprehensive, from purity levels to the recommended applications. The recombinant design ensuring human IgG2, κ provided reliable performance across different research techniques.

Q&As

How does the antibody ensure specificity toward GPNMB in various assays?

A: We designed the Anti-GPNMB Recombinant Antibody (clone Glembatumumab) with a human IgG2, κ framework to specifically bind to human GPNMB. Its performance has been validated in Western Blot, IF, IP, and ELISA assays. Detailed purity data and titration recommendations help achieve consistent results across different experiments.
What purification and validation methods are applied to this antibody?

A: We use stringent purification techniques, ensuring that the antibody exceeds 95% purity, as verified by SDS-PAGE and HPLC analyses. The antibody has been tested in multiple applications including Neutralization and Functional Assays, and the comprehensive product data provided assists researchers in effectively employing it for GPNMB detection.

View the frequently asked questions answered by Creative Biolabs Support.

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Biosimilar Overview

Please refer to Glembatumumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Glembatumumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-751.pdf

MSDS

MSDS - TAB-751.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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