Anti-Gpc3 immunotoxin (scFv)-PE

Name: Anti-Gpc3 immunotoxin (scFv)-PE
SKU: AGTO-G035E
Rating: 5 (1 reviews)

CAT#: AGTO-G035E

This immunotoxin was achieved by conjugating/fusing the Anti-Gpc3 scFv to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating liver cancer.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6316/1404_H7_2_selected.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HAP1, Hep G2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147257-GPC3

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Specifications

Host Species
Mouse

Species Reactivity
Mouse

Molecule Class
scFv-PE

Toxin Description
Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
liver cancer

Target

Alternative Names
OCI-5; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE61

Gene ID
14734

UniProt ID
Q8CFZ4

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-G035E.pdf

MSDS

MSDS - AGTO-G035E.pdf

COA

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