Recombinant Human Anti-H5N1 HA Antibody (Nb H5.3 ) (CAT#: PABL-493)
Recombinant Human Antibody (Nb H5.3 ) is capable of binding to H5N1 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H5N1 HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-H5N1 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This potently neutralizing antibody recognized a more restricted set of H5 HAs.
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SDS-PAGE
Figure 1 Anti-H5N1 HA Antibody (PABL-493) in SDS-PAGE
SDS-PAGE analysis of PABL-493 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-H5N1 HA Antibody (PABL-493) in SEC-HPLC
The purity of PABL-493 was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
ELISA
Figure 3 Anti-H5N1 HA Antibody (PABL-493) in ELISA
ELISA analysis of PABL-493 was performed by coating with Influenza A H5N1 (A/VietNam/1203/2004) Hemagglutinin Protein (HA1 Subunit) (His & Fc Tag).
Specifications
- Immunogen
- IAV haemagglutinin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native H5N1 HA
- Species Reactivity
- IAV
- Clone
- Nb H5.3
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay; Neutralization
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- HA; haemagglutinin; HA1; HA2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
| PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
| PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
| PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
| PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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