Anti-IAV HA Recombinant Antibody (HC31) (CAT#: MRO-274CT)
Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Low pH induced conformational changes of H3 rHA were inhibited by the human serum samples.
Inhibition of low pH induced H3 rHA conformation change by a convenient human serum pool (Pool) in HCCIA. H3 rHAs bound to nickel-coated plates were treated with 100 μl of 200 ng/ml trypsin to cleave HA0 into HA1 and HA2. rHA coated plates were incubated with diluent only, 1:4000, 1:400, or 1:40 diluted Pool for 1 hour. The plate was washed and treated with a range of pH buffers followed by fixation with 0.05% glutaraldehyde/PBS. An ELISA was performed using a pH-specific mAb, HC31, and detected by measuring the OD at 450 nm.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
Funcs
Figure 2 CCI showed virus neutralizing activity in TMN after removal of head-binding antibodies.
Correct folding of major epitopes for GH HA1 from A/Hong Kong/1/68 (H3N2) was confirmed by appropriate Ab binding profiles. GH HA1 from A/Hong Kong/1/68 (H3N2) and ectodomain H3 rHA from A/Aichi/1/68 (H3N2) were coated on a nickel-coated plate, an ELISA was performed by using rabbit antisera and a panel of conformation specific mAbs.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Species Reactivity
- IAV
- Clone
- HC31
- Applications
- ELISA, Funcs, IP, Neut
- Related Disease
- Influenza A infection
Applications
- Application Notes
- The ImmunoassayV HA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoprecipitation, Neutralization.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
| PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
| PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
| PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
| PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
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