Anti-IAV HA Recombinant Antibody (HC31) (CAT#: MRO-274CT)
Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Low pH induced conformational changes of H3 rHA were inhibited by the human serum samples.
Inhibition of low pH induced H3 rHA conformation change by a convenient human serum pool (Pool) in HCCIA. H3 rHAs bound to nickel-coated plates were treated with 100 μl of 200 ng/ml trypsin to cleave HA0 into HA1 and HA2. rHA coated plates were incubated with diluent only, 1:4000, 1:400, or 1:40 diluted Pool for 1 hour. The plate was washed and treated with a range of pH buffers followed by fixation with 0.05% glutaraldehyde/PBS. An ELISA was performed using a pH-specific mAb, HC31, and detected by measuring the OD at 450 nm.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
Funcs
Figure 2 CCI showed virus neutralizing activity in TMN after removal of head-binding antibodies.
Correct folding of major epitopes for GH HA1 from A/Hong Kong/1/68 (H3N2) was confirmed by appropriate Ab binding profiles. GH HA1 from A/Hong Kong/1/68 (H3N2) and ectodomain H3 rHA from A/Aichi/1/68 (H3N2) were coated on a nickel-coated plate, an ELISA was performed by using rabbit antisera and a panel of conformation specific mAbs.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Species Reactivity
- IAV
- Clone
- HC31
- Applications
- ELISA, Funcs, IP, Neut
- Related Disease
- Influenza A infection
Applications
- Application Notes
- The ImmunoassayV HA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoprecipitation, Neutralization.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
| PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
| PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
| PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
| PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
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