Anti-IAV HA Therapeutic Antibody (12D1) (CAT#: MRO-276CT)

Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).

  • Published Data
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Binding of stalk-reactive antibodies to group 2 HAs.

Binding of stalk-reactive antibodies 12D1 and CR8020 and H3 antiserum to recombinant soluble Wisc05 HA without (w/o T4 trim. domain, black lines) or with (w/T4 trim. domain, red line) trimerization domain.

Krammer, F., Margine, I., Tan, G. S., Pica, N., Krause, J. C., & Palese, P. (2012). A carboxy-terminal trimerization domain stabilizes conformational epitopes on the stalk domain of soluble recombinant hemagglutinin substrates. PloS one, 7(8), e43603.

WB

Figure 2 mAb 12D1 reacts with the LAH of HA2.

Lysates from 293T cells were transfected with pCAGGs-GFP or pCAGGs-GFP HA2 76–130, incubated with mAb 12D1, and pulled down with protein G beads. Pulled-down fractions were blotted with mAb 12D1 or rabbit anti-GFP. Arrows indicate location of the amino acid 76–130 region–GFP fusion protein. Anti-mouse HRP used to detect 12D1-binding reacts with the mouse Ig heavy and light chains within the pulled-down fraction.

Wang, T. T., Tan, G. S., Hai, R., Pica, N., Ngai, L., Ekiert, D. C., ... & Palese, P. (2010). Vaccination with a synthetic peptide from the influenza virus hemagglutinin provides protection against distinct viral subtypes. Proceedings of the National Academy of Sciences, 107(44), 18979-18984.

Neut

Figure 3 Anti-H3 mabs in microneutralization assay.

Neutralization of virus expressing the HA from either (A) A/Hong Kong/1/1968 virus or (B) A/Panama/2007/1999 virus. mAb XY102 is specific for A/HK/1968 virus. Purified mouse IgG was used for the negative control.

Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.

Funcs

Figure 4 Activity of anti-H3 mabs in plaque reduction assay on MDCK cells.

mAb 12D1 neutralize all H3 viruses tested by plaque reduction assay but not representative H1, H4 or H7 viruses. Purified mouse IgG was used for the negative control. The plaque reduction assays were performed multiple times and with each new antibody preparation. Data shown are from a single representative experiment.

Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.

IHC

Figure 5 Treatment with anti-H3 mAbs diminishes lung damage associated with viral pneumonia caused by HK68/PR8 reassortant virus.

Mice treated with mAb 12D1. 40× magnification.

Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.

Inhib

Figure 6 Red blood cell fusion assay.

Anti-H3 mabs inhibit low-pH induced fusion of HK/68 hemagglutinin with chicken red blood cells.

Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Mouse hybridoma cell line
  • Species Reactivity
  • IAV
  • Clone
  • 12D1
  • Applications
  • ELISA, WB, Neut, Funcs, IHC, Inhib
  • Related Disease
  • Influenza A infection

Applications

  • Application Notes
  • The ImmunoassayV HA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Neutralization, Functional Assay, Immunohistochemistry, Inhibition.

Target

  • Alternative Names
  • HA; hemagglutinin; HA1; HA2

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "HA"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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