Anti-IAV HA Recombinant Antibody (39A4) (CAT#: MRO-278CT)
Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).
Specific Inquiry
WB
Figure 1 MAbs react with H3 hemagglutinin by western blot.
MAbs 7A7, 12D1 and 39A4 react with the A/HK/1/1968 hemagglutinin under non-reducing conditions. MAbs 7A7 (lane 1) and 39A4 (lane 3) bind HA trimer complexes.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
ELISA
Figure 2 Reactivity of anti-H3 mAbs by ELISA.
mAbs react with purified A/Alabama/1981 (H3) virus. mAb XY102 is specific for the hemagglutinin of A/HK/1968 virus.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
IHC
Figure 3 Treatment with anti-H3 mAbs diminishes lung damage associated with viral pneumonia caused by HK68/PR8 reassortant virus.
Untreated mice treated with mAb 39A4. 40× magnification.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
Neut
Figure 4 Anti-H3 mabs in microneutralization assay.
Neutralization of virus expressing the HA from either (A) A/Hong Kong/1/1968 virus or (B) A/Panama/2007/1999 virus. mAb XY102 is specific for A/HK/1968 virus. Purified mouse IgG was used for the negative control.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
Funcs
Figure 5 Activity of anti-H3 mabs in plaque reduction assay on MDCK cells.
mAb 39A4 neutralize all H3 viruses tested by plaque reduction assay but not representative H1, H4 or H7 viruses. Purified mouse IgG was used for the negative control. The plaque reduction assays were performed multiple times and with each new antibody preparation. Data shown are from a single representative experiment.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
Inhib
Figure 6 Red blood cell fusion assay.
Anti-H3 mabs inhibit low-pH induced fusion of HK/68 hemagglutinin with chicken red blood cells.
Wang, T. T., Tan, G. S., Hai, R., Pica, N., Petersen, E., Moran, T. M., & Palese, P. (2010). Broadly protective monoclonal antibodies against H3 influenza viruses following sequential immunization with different hemagglutinins. PLoS pathogens, 6(2), e1000796.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Species Reactivity
- IAV
- Clone
- 39A4
- Applications
- ELISA, WB, Neut, Funcs, IHC, Inhib
- Related Disease
- Influenza A infection
Applications
- Application Notes
- The ImmunoassayV HA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Neutralization, Functional Assay, Immunohistochemistry, Inhibition.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single Domain Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-203 | Recombinant Human Anti-IAV HA Antibody (3.1) | WB, ELISA, FuncS | IgG |
| PABL-204 | Recombinant Human Anti-IAV HA Antibody (39.29) | Neut, FuncS | IgG |
| PABL-205 | Recombinant Human Anti-IAV HA Antibody (1957) | WB, Neut, FuncS | IgG |
| PABL-206 | Recombinant Human Anti-IAV HA Antibody (1F1) | WB, ELISA, FuncS | IgG |
| PABL-207 | Recombinant Human Anti-IAV HA Antibody (5J8) | FuncS | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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