Recombinant Mouse Antibody (19D9D6) is capable of binding to HCV, expressed in Chinese Hamster Ovary cells (CHO). The core peptide corresponding to residues 13–40 is recognized by 19D9D6 with an affinity as strong as 2–45 in BIAcore and shows a classical and specific behavior in competition ELISA against core 1–120 against which it was raised.
Figure 1 Competition ELISA between peptides.
The inhibition curve for 13–40 (continuous line) shows that the shorter peptide out-competes the longer one (2–45; broken line) by a factor of 10–50. The two peptides have a similar behavior at low peptide concentrations (in the range 10⁻¹²– 10⁻⁸ ), but at higher peptide concentrations, 2–45 is a less effective competitor than 13–40, possibly due to some degree of aggregation (to be verified by other techniques) of the 2–45 peptide.
Ménez, R., Bossus, M., Muller, B. H., Sibaï, G., Dalbon, P., Ducancel, F., ... & Stura, E. A. (2003). Crystal structure of a hydrophobic immunodominant antigenic site on hepatitis C virus core protein complexed to monoclonal antibody 19D9D6. The Journal of immunology, 170(4), 1917-1924.
Figure 2 Western blot of purified and delipidated LVP.
Ether-butanol-treated LVP corresponding to 3 × 10⁶ HCV RNA copies (lane a), HepG2 cells (negative control) (lane b), and HepG2 cells stably transfected with HCV core cDNA (positive control) (lane c) are shown. Detection of HCV core protein was performed with monoclonal antibody 19D9D6.
Andre, P., Komurian-Pradel, F., Deforges, S., Perret, M., Berland, J. L., Sodoyer, M., ... & Lotteau, V. (2002). Characterization of low-and very-low-density hepatitis C virus RNA-containing particles. Journal of virology, 76(14), 6919-6928.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-444-S(P) | Recombinant Anti-HCV Antibody scFv Fragment | WB, IHC, FuncS | scFv |
HPAB-N0132-YC-S(P) | Recombinant Human Anti-HCV Antibody scFv Fragment (TRN-1002) | ELISA, Neut | Human scFv |
FAMAB-0024WJ-S(P) | Human Anti-HCV Recombinant Antibody (clone B12.F8); scFv Fragment | ELISA, IF, IHC | Human scFv |
FAMAB-0025WJ-S(P) | Human Anti-HCV Recombinant Antibody (clone CM3.B6); scFv Fragment | ELISA, IF, IHC | Human scFv |
FAMAB-0026WJ-S(P) | Human Anti-HCV Recombinant Antibody (clone E6.D10); scFv Fragment | ELISA, IF, IHC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-079 | Recombinant Anti-HCV VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-444 | Recombinant Human Anti-HCV Antibody | IF, Neut, FuncS | IgG |
HPAB-0232-FY | Recombinant Human Anti-HCV Antibody (TRN1007) | WB, F, N | Human IgG |
HPAB-0233-FY | Recombinant Human Anti-HCV Antibody (TRN1009) | WB, E, N | Human IgG |
FAMAB-0585WJ | Human Anti-HCV Recombinant Antibody (clone 42C) | ELISA, WB, Neut | Human IgG |
VS3-WK1330 | Mouse Anti-HCV Recombinant Antibody (VS3-WK1330) | ELISA | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-444-F(E) | Recombinant Human Anti-HCV Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
HPAB-0232-FY-F(E) | Recombinant Human Anti-HCV Antibody Fab Fragment (TRN1007) | WB, F, N | Human Fab |
HPAB-0233-FY-F(E) | Recombinant Human Anti-HCV Antibody Fab Fragment (TRN1009) | WB, E, N | Human Fab |
HPAB-1178-FY-S(P) | Recombinant Anti-HCV Antibody Fab Fragment (83-128) | ELISA | Human Fab |
HPAB-1179-FY-S(P) | Recombinant Anti-HCV Antibody Fab Fragment (95-2) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-344CL | Human Anti-HCV Recombinant Antibody (TAB-344CL) | FuncS | Human IgG |
PABX-071 | Recombinant Human Anti-HCV Antibody (HC33.4) | WB, ELISA, Neut, FuncS | IgG |
PABX-079 | Recombinant Human Anti-Hepatitis C virus Antibody (AP33) | WB, ELISA, FuncS | IgG |
PABX-071-F (E) | Recombinant Human Anti-HCV Antibody Fab Fragment (HC33.4) | WB, ELISA, Neut, FuncS | Fab |
PABX-079-F (E) | Recombinant Human Anti-Hepatitis C virus Antibody Fab Fragment (AP33) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-071-S (P) | Recombinant Human Anti-HCV Antibody scFv Fragment (HC33.4) | WB, ELISA, Neut, FuncS | scFv |
PABX-079-S (P) | Recombinant Human Anti-Hepatitis C virus Antibody scFv Fragment (AP33) | WB, ELISA, FuncS | scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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