Recombinant Mouse Anti-HCV Antibody (19D9D6) (CAT#: PABZ-057)

Recombinant Mouse Antibody (19D9D6) is capable of binding to HCV, expressed in Chinese Hamster Ovary cells (CHO). The core peptide corresponding to residues 13–40 is recognized by 19D9D6 with an affinity as strong as 2–45 in BIAcore and shows a classical and specific behavior in competition ELISA against core 1–120 against which it was raised.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Competition ELISA between peptides.

Figure 1 Competition ELISA between peptides.

The inhibition curve for 13–40 (continuous line) shows that the shorter peptide out-competes the longer one (2–45; broken line) by a factor of 10–50. The two peptides have a similar behavior at low peptide concentrations (in the range 10⁻¹²– 10⁻⁸ ), but at higher peptide concentrations, 2–45 is a less effective competitor than 13–40, possibly due to some degree of aggregation (to be verified by other techniques) of the 2–45 peptide.

Ménez, R., Bossus, M., Muller, B. H., Sibaï, G., Dalbon, P., Ducancel, F., ... & Stura, E. A. (2003). Crystal structure of a hydrophobic immunodominant antigenic site on hepatitis C virus core protein complexed to monoclonal antibody 19D9D6. The Journal of immunology, 170(4), 1917-1924.

Figure 2 Western blot of purified and delipidated LVP.

Figure 2 Western blot of purified and delipidated LVP.

Ether-butanol-treated LVP corresponding to 3 × 10⁶ HCV RNA copies (lane a), HepG2 cells (negative control) (lane b), and HepG2 cells stably transfected with HCV core cDNA (positive control) (lane c) are shown. Detection of HCV core protein was performed with monoclonal antibody 19D9D6.

Andre, P., Komurian-Pradel, F., Deforges, S., Perret, M., Berland, J. L., Sodoyer, M., ... & Lotteau, V. (2002). Characterization of low-and very-low-density hepatitis C virus RNA-containing particles. Journal of virology, 76(14), 6919-6928.


Specifications

  • Immunogen
  • HCV Hepatitis C virus
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native HCV
  • Species Reactivity
  • HCV
  • Clone
  • 19D9D6
  • Applications
  • ELISA 0.15 nM
    WB 1:1000-1:5000

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody 19D9D6 has been reported in applications of ELISA, WB. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
    ELISA: A reported concentration of the antibody 19D9D6 for ELISA was 0.15 nM.

Target

  • Alternative Names
  • Hepatitis C virus; HCV; HVC; Flaviviridae

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 19D9D6"

See other products for "HCV"

Single-domain Antibody

CAT Product Name Application Type
NABG-079 Recombinant Anti-HCV VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH

Mouse Antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABZ-057. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare