Recombinant Human Anti-Hepatitis C virus Antibody (AP33)
CAT#: PABX-079
Anti-Hepatitis C virus antibody is a Human antibody of IgG class that binds to an Hepatitis C virus.
Published Data
Figure 1 Analysis of E1E2 of diverse HCV genotypes.
Radiolabeled proteins in the lysate of HEK 293T cells cotransfected with different plasmids as described in A above were immunoprecipitated using a mixture of anti-E2 MAbs AP33 and ALP98. The immune complexes were analyzed by SDS-10% PAGE under reducing conditions. Positions of protein size markers are shown (in kDa).
Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F. L., ... & Patel, A. H. (2005). Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein. Journal of virology, 79(17), 11095-11104.
Figure 2 Lysine residues upstream of the conserved epitope 412-423 are important for mAb AP33 binding.
The binding pattern of mAb 3/11 and AP33 was compared using wild type or mutated patientderived peptides spanning AA region 406-427. Serial dilutions of mAbs were incubated with surface-attached peptides (25 lg/mL), and bound antibodies were detected with a horseradish peroxidase–conjugated antispecies secondary antibody. Peptides were generated with K- or A-substitutions at positions 408 and/or 410 (H77c: K408A, N410K, N410A, and double mutants K408A-N410K and K408A-N410A; LT-P05_VF: T408A, T408K, K410A, and double mutants T408K-K410A and T408A-K410A; CC-P08_VA: A408K, K410A, and double mutant A408KK410A). The resulting peptide sequences are shown and changed AA marked. Dots indicate AA similarity.
Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.
Figure 3 mAb AP33 protects humanized mice against an HCV gt1b challenge.
Chimeric animals were challenged with 104 IU of HCV RNA of mLT_P05 without pretreatment (no IgG) or after passive immunization on day 23 with 1 mg of mAb 3/11 or AP33. Plasma HCV-RNA levels (IU/mL) were measured until 8 weeks after viral challenge. Mice that remained noninfected postinoculation are shown as HCV RNA <750 IU/mL (LOQ, dotted line). Animal B338RL died 4 weeks after viral challenge.
Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Hepatitis C virus Hepatitis C virus
- Species Reactivity
- Hepatitis C virus
- Clone
- AP33
- Applications
- WB 1:1000-1:5000
ELISA 1:10000-1:20000
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Hepatitis C virus; HCV; HVC; Flaviviridae
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Customer Review
There are currently no Customer reviews or questions for PABX-079. Click the button above to contact us or submit your feedback about this product.
Laura Hill14.Feb,23
Excellent for Hepatitis C Research
The Anti-Hepatitis C Virus Antibody (AP33) has been excellent for our hepatitis C research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
John Martinez05.Oct,22
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for hepatitis C virus is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
Sarah Evans31.Jul,21
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for hepatitis C virus have been particularly beneficial.
Q&As
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Is the anti-Hepatitis C virus antibody AP33 suitable for use in Western blotting?
A: Yes, the anti-Hepatitis C virus antibody AP33 (PABX-079) is suitable for use in Western blotting. It provides specific binding to the HCV E2 protein, allowing for reliable detection in Western blot assays.
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What are the storage recommendations for the anti-Hepatitis C virus antibody AP33?
A: The recommended storage condition for the anti-Hepatitis C virus antibody AP33 (PABX-079) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.
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Can the anti-Hepatitis C virus antibody AP33 be used in immunoprecipitation assays?
A: Yes, the anti-Hepatitis C virus antibody AP33 (PABX-079) can be used in immunoprecipitation assays. It provides specific binding to the HCV E2 protein, enabling the successful precipitation of the target protein from complex mixtures.
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Is the anti-Hepatitis C virus antibody AP33 effective in ELISA applications?
A: Yes, the anti-Hepatitis C virus antibody AP33 (PABX-079) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of the HCV E2 protein.
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What is the optimal dilution for using the anti-Hepatitis C virus antibody AP33 in immunofluorescence?
A: The optimal dilution for using the anti-Hepatitis C virus antibody AP33 (PABX-079) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.
View the frequently asked questions answered by Creative Biolabs Support.
Citations
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Deng, Kai, et al. "Hepatitis C virus hypervariable region 1 antibodies interrupt E2-SR-B1 interaction to suppress viral infection." Iscience 26.4 (2023). https://doi.org/10.1016/j.isci.2023.106421This study investigates the role of antibodies targeting the hypervariable region 1 (HVR1) of the hepatitis C virus (HCV) envelope protein in blocking viral infection. The researchers demonstrated that antibodies specific to the HVR1 region could inhibit the interaction between the HCV E2 protein and the SR-B1 receptor, which is crucial for viral entry into host cells. Through experiments with antibodies derived from chronic hepatitis C (CHC) patients and rabbits immunized with HVR1 peptides, the study showed that these antibodies could effectively neutralize multiple HCV genotypes, thereby highlighting the potential of HVR1-targeting antibodies in controlling HCV infections.
Creative Biolabs provided the monoclonal antibody AP33 (Cat# PABX-079) used to block cell-free transmission of HCV in the cell-cell transmission assays. The AP33 antibody was crucial in demonstrating the efficacy of patient-derived IgGs and HVR1-specific antibodies in inhibiting both cell-free and cell-cell transmission of the virus. This contribution was essential for validating the study's findings on the role of HVR1-targeting antibodies in preventing HCV infection and transmission.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-079, RRID: AB_3111698)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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