Recombinant Human Anti-Hepatitis C virus  Antibody Fab Fragment (AP33) (CAT#: PABX-079-F (E))

Figure 1 Analysis of E1E2 of diverse HCV genotypes.

Radiolabeled proteins in the lysate of HEK 293T cells cotransfected with different plasmids as described in A above were immunoprecipitated using a mixture of anti-E2 MAbs AP33 and ALP98. The immune complexes were analyzed by SDS-10% PAGE under reducing conditions. Positions of protein size markers are shown (in kDa).

Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F. L., ... & Patel, A. H. (2005). Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein. Journal of virology, 79(17), 11095-11104.

Figure 2 Lysine residues upstream of the conserved epitope 412-423 are important for mAb AP33 binding.

The binding pattern of mAb 3/11 and AP33 was compared using wild type or mutated patientderived peptides spanning AA region 406-427. Serial dilutions of mAbs were incubated with surface-attached peptides (25 lg/mL), and bound antibodies were detected with a horseradish peroxidase–conjugated antispecies secondary antibody. Peptides were generated with K- or A-substitutions at positions 408 and/or 410 (H77c: K408A, N410K, N410A, and double mutants K408A-N410K and K408A-N410A; LT-P05_VF: T408A, T408K, K410A, and double mutants T408K-K410A and T408A-K410A; CC-P08_VA: A408K, K410A, and double mutant A408KK410A). The resulting peptide sequences are shown and changed AA marked. Dots indicate AA similarity.

Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.

Figure 3 mAb AP33 protects humanized mice against an HCV gt1b challenge.

Chimeric animals were challenged with 104 IU of HCV RNA of mLT_P05 without pretreatment (no IgG) or after passive immunization on day 23 with 1 mg of mAb 3/11 or AP33. Plasma HCV-RNA levels (IU/mL) were measured until 8 weeks after viral challenge. Mice that remained noninfected postinoculation are shown as HCV RNA <750 IU/mL (LOQ, dotted line). Animal B338RL died 4 weeks after viral challenge.

Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.