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Rabbit Anti-HDGF Recombinant Antibody (clone R78-2C-3) (CAT#: VS3-FY2147)

This R78-2C-3 recombinant monoclonal antibody specifically binds to human HDGF protein, a calculated weight of 27 kDa. It cross-reacts with mouse. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), flow cytometry (dilution 1/50).


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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53422/if_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26035/if_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_B_7_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_3.jpg
Figure 6 Liver
Figure 6 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_4.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_5.jpg
Figure 8 Testis
Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_6_6.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143321-HDGF

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Mouse HDGF
  • Species Reactivity
  • Human, Mouse
  • Clone
  • R78-2C-3
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Flow Cytometry
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 37kDa.
  • Related Disease
  • Lung Cancer and Hepatocellular Carcinoma

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200
    Flow Cytometry: 1/50

Target

  • Alternative Names
  • Heparin Binding Growth Factor; High Mobility Group Protein 1-Like 2; Hepatoma Derived Growth Factor; HMG1L2; Hepatoma-Derived Growth Factor (High-Mobility Group Protein 1-Like); High-Mobility Group Protein 1-Like; Hepatoma-Derived Growth Factor; HMG-1L2
  • Sequence Similarities
  • Belongs to the HDGF family.
  • Cellular Localization
  • Cytoplasm, Nucleus, Secreted
  • Post Translation Modifications
  • Sumoylated with SUMO1.
    Sumoylation prevents binding to chromatin.
    (P51858-HDGF_HUMAN)
    Phosphorylation at Ser-165 is likely to be required for secretion. (P51858-HDGF_HUMAN)
    Ubiquitination at Lys11 and Lys80 (NX_P51858 [NX_P51858-1])
    Modification sites at PhosphoSitePlus (P51858)
    Glycosylation from GlyGen (P51858) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Acts as a transcriptional repressor.
    Has mitogenic activity for fibroblasts.
    Heparin-binding protein.

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "HDGF"

Recombinant Antibody

CAT Product Name Application Type
MOB-2467z Mouse Anti-HDGF Recombinant Antibody (clone 33H7) ELISA, ICC, IF, IHC, WB Mouse IgG2a
MOB-0146CT Recombinant Mouse anti-Human HDGF Monoclonal antibody (37D5b) Dot, WB
HPAB-0032-LSX Mouse Anti-HDGF Recombinant Antibody (clone 2F12) ELISA, WB, IP, IF, ICC Mouse IgG1, κ
HPAB-0033-LSX Mouse Anti-HDGF Recombinant Antibody (clone H3) ELISA, WB, RIA, FC, ICC, IHC, FuncS Mouse IgG1
HPAB-0034-LSX Mouse Anti-HDGF Recombinant Antibody (clone C1) ELISA, WB, RIA, FC, ICC, IHC, FuncS Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1581 Rabbit Anti-HDGF Recombinant Antibody (clone DS1581AB) WB, IHC, ICC, IP, FC Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0032-LSX-S(P) Mouse Anti-HDGF Recombinant Antibody (clone 2F12); scFv Fragment ELISA, WB, IP, IF, ICC Mouse scFv
HPAB-0033-LSX-S(P) Mouse Anti-HDGF Recombinant Antibody (clone H3); scFv Fragment ELISA, WB, RIA, FC, ICC Mouse scFv
HPAB-0034-LSX-S(P) Mouse Anti-HDGF Recombinant Antibody (clone C1); scFv Fragment ELISA, WB, RIA, FC, ICC Mouse scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0032-LSX-F(E) Mouse Anti-HDGF Recombinant Antibody (clone 2F12); Fab Fragment ELISA, WB, IP, IF, ICC Mouse Fab
HPAB-0033-LSX-F(E) Mouse Anti-HDGF Recombinant Antibody (clone H3); Fab Fragment ELISA, WB, RIA, FC, ICC Mouse Fab
HPAB-0034-LSX-F(E) Mouse Anti-HDGF Recombinant Antibody (clone C1); Fab Fragment ELISA, WB, RIA, FC, ICC Mouse Fab

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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