Rabbit Anti-HDGF Recombinant Antibody (clone R78-2C-3)
CAT#: VS3-FY2147
This R78-2C-3 recombinant monoclonal antibody specifically binds to human HDGF protein, a calculated weight of 27 kDa. It cross-reacts with mouse. We recommend antibody dilution: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), flow cytometry (dilution 1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53422/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26035/if_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48728/138401_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143321-HDGF
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse HDGF
- Species Reactivity
- Human, Mouse
- Clone
- R78-2C-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 37kDa.
- Related Disease
- Lung Cancer and Hepatocellular Carcinoma
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Flow Cytometry: 1/50
Target
- Alternative Names
- Heparin Binding Growth Factor; High Mobility Group Protein 1-Like 2; Hepatoma Derived Growth Factor; HMG1L2; Hepatoma-Derived Growth Factor (High-Mobility Group Protein 1-Like); High-Mobility Group Protein 1-Like; Hepatoma-Derived Growth Factor; HMG-1L2
- Gene ID
- 3068
- UniProt ID
- P51858
- Sequence Similarities
- Belongs to the HDGF family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- Sumoylated with SUMO1.
Sumoylation prevents binding to chromatin.
(P51858-HDGF_HUMAN)
Phosphorylation at Ser-165 is likely to be required for secretion. (P51858-HDGF_HUMAN)
Ubiquitination at Lys11 and Lys80 (NX_P51858 [NX_P51858-1])
Modification sites at PhosphoSitePlus (P51858)
Glycosylation from GlyGen (P51858) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011507755.2; XP_011507756.1; XP_011507757.1; XP_047274787.1; XP_047274788.1
- Function
- Acts as a transcriptional repressor.
Has mitogenic activity for fibroblasts.
Heparin-binding protein.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HDGF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2467z | Mouse Anti-HDGF Recombinant Antibody (clone 33H7) | ELISA, ICC, IF, IHC, WB | Mouse IgG2a |
| MOB-0146CT | Recombinant Mouse anti-Human HDGF Monoclonal antibody (37D5b) | Dot, WB | |
| HPAB-0032-LSX | Mouse Anti-HDGF Recombinant Antibody (clone 2F12) | ELISA, WB, IP, IF, ICC | Mouse IgG1, κ |
| HPAB-0033-LSX | Mouse Anti-HDGF Recombinant Antibody (clone H3) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| HPAB-0034-LSX | Mouse Anti-HDGF Recombinant Antibody (clone C1) | ELISA, WB, RIA, FC, ICC, IHC, FuncS | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1581 | Rabbit Anti-HDGF Recombinant Antibody (clone DS1581AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); scFv Fragment | ELISA, WB, IP, IF, ICC | Mouse scFv |
| HPAB-0033-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone H3); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| HPAB-0034-LSX-S(P) | Mouse Anti-HDGF Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB, RIA, FC, ICC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0032-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone 2F12); Fab Fragment | ELISA, WB, IP, IF, ICC | Mouse Fab |
| HPAB-0033-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone H3); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| HPAB-0034-LSX-F(E) | Mouse Anti-HDGF Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB, RIA, FC, ICC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY121 | AbPlus™ Anti-HDGF Magnetic Beads (2F12) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1007 | Anti-HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3107 | Anti-Mouse HDGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3106 | Anti-Human HDGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC140 | Recombinant Anti-HDGF Eliminating Antibody, pH-Sensitive (H3 var.) | Antigen-Sweeping In Vivo. | Mouse IgG1 |
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