Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone NC-1) (CAT#: PABL-562)

Figure 1 Binding of MAb NC-1 to peptides and peptide complexes using a dot blot assay.

The binding of the antisera (1:100) and MAb (10 mg/ml) to the individual peptides and peptide complexes was detected by dot blot assay. The complex N-36/C-34 was prepared by mixing peptides N-36 and C-34 in PBS at equimo- lar concentrations (2 mM).

Jiang, S., Lin, K., Zhang, L., & Debnath, A. K. (1999). A screening assay for antiviral compounds targeted to the HIV-1 gp41 core structure using a conformation-specific monoclonal antibody. Journal of virological methods, 80(1), 85-96.

Figure 2 Binding of NC-1 to the model polypeptides containing N and C peptides by ELISA analysis.

Each of the N51(L6)C43, N36(L6)C34, and N34(L6)C28 polypeptides (∼1 μM) were used to coat wells of a microplate. The binding of the purified NC-1 IgG at the indicated concentrations to each coated polypeptide was tested by ELISA.

Jiang, S., Lin, K., & Lu, M. (1998). A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein. Journal of virology, 72(12), 10213-10217.

Figure 3 Binding of MAb NC-1 to transmembrane glycoproteins expressed on HIV-infected cells by flow cytometric analysis.

HIV-1IIIB-infected cells were reacted with the MAbs NC-1 (A) and 2F5 (B) (5 μg of IgG/ml) in the presence or absence of sCD4 (10 μg/ml) and analyzed by flow cytometry. Binding of NC-1 to HIV-2ROD-infected cells was also tested (C). Normal mouse (NM) IgG was used as a control.

Jiang, S., Lin, K., & Lu, M. (1998). A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein. Journal of virology, 72(12), 10213-10217.