Mouse Anti-HRAS Recombinant Antibody (clone 2H12)

Name: Mouse Anti-HRAS Recombinant Antibody (clone 2H12)
SKU: MOB-1684z
Rating: 5 (1 reviews)

CAT#: MOB-1684z

This product is a mouse antibody that recognizes human HRAS. It can be used in the following applications: WB, IF, IHC.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70222/1357_B12_1_selected.jpg

Normal Tissue Figure 2 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2015/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_B_9_5.jpg

Normal Tissue Figure 4 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_8_3.jpg

Normal Tissue Figure 5 Gallbladder

Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_5_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_9_8.jpg

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174775-HRAS

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Specifications

Immunogen
Human Harvey Rat Sarcoma Viral Oncogene Homolog

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG1

Antibody Isotype
IgG1

Specificity
Human HRAS

Species Reactivity
Human

Clone
2H12

Applications
WB, IF, IHC

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Western blot, Immunofluorescence, Immunohistochemistry.

Target

Alternative Names
HRAS; HAMSV; P21ras; C-BAS/HAS; H-RASIDX; C-HA-RAS1; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; P19 H-RasIDX Protein; Transforming Protein P21; Harvey Rat Sarcoma Viral Oncoprotein; C-H-RAS; RASH1; Transformation Gene: Oncogene HAMSV; Ha-Ras1 Pro

UniProt ID
P01112

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(Creative Biolabs Cat# MOB-1684z, RRID: AB_3713951)

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Related Diseases

Bladder Cancer

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-1684z.pdf

MSDS

MSDS - MOB-1684z.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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