Recombinant Mouse anti-Human DNMT1 Monoclonal antibody (EML149)
CAT#: MOB-0562CT
Recombinant Mouse monoclonal antibody to Human DNMT1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
Normal Tissue
Figure 4 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 1-111 of Human Dnmt1
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML149
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.20 -0.5 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- DNMT1; DNA (cytosine-5-)-methyltransferase 1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9;
- Gene ID
- 1786
- UniProt ID
- P26358
- Protein Refseq
- NP_001124295
- Function
- DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; metal ion binding; protein binding; transcription factor binding; transferase activity; zinc ion binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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