Mouse Anti-DNMT1 Recombinant Antibody (clone 60B1220.1)
CAT#: VS-0725-FY209
The Mouse Anti-DNMT1 Recombinant Antibody (clone 60B1220.1) is a recombinant antibody targeting a peptide corresponding to amino acid residues 637-651 of human DNMT1. It is suitable for Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Immunoprecipitation (IP), and Western Blot (WB). DNMT1 is the major enzyme maintaining DNA methylation patterns after replication, crucial for mammalian epigenetic gene regulation. This antibody is a valuable tool for studying DNA methylation and its role in development and disease.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5876/613_E12_1_red_green.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_4_6.jpg
Normal Tissue
Figure 4 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2694/8662_A_1_7.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130816-DNMT1
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Specifications
- Immunogen
- A peptide corresponding to amino acid residues 637-651 of human DNMT1.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human DNMT1
- Species Reactivity
- Human, Mouse
- Clone
- 60B1220.1
- Applications
- Chromatin Immunoprecipitation, Immunohistochemistry, Immunoprecipitation, Western Blot
- Related Disease
- Narcolepsy, Autosomal Dominant
Product Property
- Purification
- Affinity purified
- Molecular Weight
- The molecular weight of the antigen is 190 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 2-4 µg/mL
Target
- Alternative Names
- DNA Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; CXXC9
- Gene ID
- 1786
- UniProt ID
- P26358
- Long Name
- DNA Methyltransferase 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys58, Lys88, Lys134, Lys586, Lys666, Lys928, Lys961, Lys981, Lys997, Lys1323, Lys1415, Lys1483, and Lys1572
Modification
Glycosylation from GlyGen (P26358) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001124295.1; XP_047278844.1
- Function
- Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DNMT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0164MZ | Chicken Anti-DNMT1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1028 | Hi-Affi™ Rabbit Anti-DNMT1 Recombinant Antibody (clone DS1028AB) | FC, ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0486-CN | Rabbit Anti-DNMT1 Recombinant Antibody (clone JF09-89) | WB, IF, IHC | Rabbit IgG |
| MRO-A1926-YJ | Mouse Anti-DNMT1 Recombinant Antibody (clone CBS388) | WB, ELISA | Mouse IgG2b |
| ZG-0083U | Rabbit Anti-DNMT1 Recombinant Antibody (clone 1A9) | ELISA, IHC, IF | Rabbit IgG |
| VS3-XY3334 | Rabbit Anti-DNMT1 Recombinant Antibody (clone R08-4C7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS13-YC315 | Rabbit Anti-DNMT1 Recombinant Antibody (VS13-YC315) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC314 | CytoStream™ Mouse Anti-DNMT1 Recombinant Antibody (VS13-YC314) | WB, IHC-P, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2045 | Anti-DNMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2046 | Anti-Mouse DNMT1 Immunohistochemistry Kit | IHC |
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